SHP1 ELISA 试剂盒
PTPN6
适用: 小鼠
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all SHP1 (PTPN6) ELISA试剂盒
- SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse PTPN6 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse PTPN6.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product PTPN6 ELISA Kit
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Protein-tyrosine Phosphatase 1C (PTPN6) ELISA Kit
PTPN6 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all SHP1 (PTPN6) ELISA试剂盒
- SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))
- 别名
- PTPN6 (PTPN6 产品)
- 别名
- HCPH ELISA Kit, HPTP1C ELISA Kit, PTP-1C ELISA Kit, SH-PTP1 ELISA Kit, SHP-1 ELISA Kit, SHP-1L ELISA Kit, SHP1 ELISA Kit, DRPLA ELISA Kit, Hcph ELISA Kit, Ptp1C ELISA Kit, me ELISA Kit, motheaten ELISA Kit, Ptph6 ELISA Kit, Shp-1 ELISA Kit, fj22f05 ELISA Kit, wu:fj22f05 ELISA Kit, zgc:55501 ELISA Kit, ptpn6 ELISA Kit, protein tyrosine phosphatase, non-receptor type 6 ELISA Kit, protein tyrosine phosphatase, non-receptor type 6 L homeolog ELISA Kit, PTPN6 ELISA Kit, Ptpn6 ELISA Kit, ptpn6 ELISA Kit, ptpn6.L ELISA Kit
- UniProt
- P29351
- 途径
- JAK/STAT Signaling, TCR Signaling, TLR signaling, Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, BCR Signaling
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