MTOR ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for MTOR ELISA 试剂盒 (ABIN7830800)
抗原
See all MTOR (mTOR) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
-
-
原理
- MTOR (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MTOR expression in cultured cells.
-
Analytical Method
- Qualitative
-
交叉反应
- 小鼠, 大鼠
-
-
Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) ELISA Kit
mTOR 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) ELISA KitmTOR 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) ELISA KitmTOR 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) ELISA KitmTOR 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) ELISA KitmTOR 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) ELISA KitmTOR 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
板类型
- Uncoated
-
实验流程
-
- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C
-
储存方法
- Store the kit at 4°C.
-
-
- MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))
-
别名
- MTOR
-
背景
-
Synonyms: FRAP|FRAP1|FRAP2|RAFT1|RAPT1
Gene Description: mechanistic target of rapamycin
-
基因ID
- 2475
-
途径
- PI3K-Akt Signaling, RTK signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effect
抗原 See all MTOR (mTOR) ELISA试剂盒
-
