c-MET ELISA 试剂盒
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Quick Overview for c-MET ELISA 试剂盒 (ABIN7830760)
抗原
See all c-MET (MET) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- MET (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MET expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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Met Proto-Oncogene (MET) ELISA Kit
MET 适用: 人, 小鼠, 大鼠 pTyr1234 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Met Proto-Oncogene (MET) IQ-ELISA KitMET 适用: 人 qPCR Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
Met Proto-Oncogene (MET) ELISA KitMET 适用: 人, 小鼠, 大鼠 pTyr1234 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Met Proto-Oncogene (MET) ELISA KitMET 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Met Proto-Oncogene (MET) ELISA KitMET 适用: 人 AA 25-307 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Met Proto-Oncogene (MET) ELISA KitMET 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Met Proto-Oncogene (MET) ELISA KitMET 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- c-MET (MET) (Met Proto-Oncogene (MET))
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别名
- MET
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背景
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Synonyms: AUTS9|HGFR|RCCP2|c-Met
Gene Description: met proto-oncogene (hepatocyte growth factor receptor)
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基因ID
- 4233
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UniProt
- P08581
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途径
- RTK signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
抗原 See all c-MET (MET) ELISA试剂盒
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