c-MET ELISA 试剂盒
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北京 101111
Quick Overview for c-MET ELISA 试剂盒 (ABIN6720214)
抗原
See all c-MET (MET) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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Supplier Product No.
- koa0436
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Supplier
- Rockland
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原理
- Human C-MET - HGFR Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: E25-R307 (alpha) & S308-T932 (beta) -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 5 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Met Proto-Oncogene (MET) ELISA Kit
MET 适用: 人, 小鼠, 大鼠 pTyr1234 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Met Proto-Oncogene (MET) ELISA KitMET 适用: 人, 小鼠, 大鼠 pTyr1234 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Met Proto-Oncogene (MET) IQ-ELISA KitMET 适用: 人 qPCR Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
Met Proto-Oncogene (MET) ELISA KitMET 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Met Proto-Oncogene (MET) ELISA KitMET 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Met Proto-Oncogene (MET) ELISA KitMET 适用: 犬 Colorimetric Sandwich ELISA 156.0 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Met Proto-Oncogene (MET) ELISA KitMET 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Met Proto-Oncogene (MET) ELISA KitMET 适用: 人 AA 25-307 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Met Proto-Oncogene (MET) ELISA KitMET 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
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说明
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Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human C-MET standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human C-MET standard solution and each sample be measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 4.1%, 5.2%, 6.1%
Inter-Assay Precision (CV%): 5.3%, 6.1%, 6.9% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- c-MET (MET) (Met Proto-Oncogene (MET))
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别名
- C-MET/HGFR
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背景
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Background: C-Met (MET or MNNG HOS Transforming gene) is a proto-oncogene that encodes a protein known as hepatocyte growth factor receptor (HGFR). MET proto-oncogene has a total length of 125,982 bp, and it is located in the 7q31 locus of chromosome 7. MET is a membrane receptor that is essential for embryonic development and wound healing. Activation of MET triggers mitogenesis, and morphogenesis.
Gene Full Name: MET proto-oncogene, receptor tyrosine kinase
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基因ID
- 4233
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NCBI登录号
- NP_000236
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UniProt
- P08581
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途径
- RTK signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
抗原 See all c-MET (MET) ELISA试剂盒
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