MAP2K7 ELISA 试剂盒
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Quick Overview for MAP2K7 ELISA 试剂盒 (ABIN7830743)
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检测方法
实验类型
应用范围
样品类型
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原理
- MAP2K7 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MAP2K7 expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7) ELISA Kit
MAP2K7 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7) ELISA KitMAP2K7 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- MAP2K7 (Mitogen-Activated Protein Kinase Kinase 7 (MAP2K7))
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别名
- MAP2K7
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背景
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Synonyms: Jnkk2|MAPKK7|MKK7|PRKMK7
Gene Description: mitogen-activated protein kinase kinase 7
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基因ID
- 5609
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UniProt
- O14733
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途径
- MAPK Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, BCR Signaling
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