EGFR ELISA 试剂盒
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Quick Overview for EGFR ELISA 试剂盒 (ABIN7830618)
抗原
See all EGFR ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- EGFR (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of EGFR expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- EGFR (Epidermal Growth Factor Receptor (EGFR))
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别名
- EGFR
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背景
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Synonyms: ERBB|ERBB1|HER1|PIG61|mENA
Gene Description: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
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基因ID
- 1956
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UniProt
- P00533
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途径
- NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins
抗原 See all EGFR ELISA试剂盒
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