E2F1 ELISA 试剂盒
人 E2F1 ELISA Kit, Colorimetric is an assay for quantification of 人 E2F1.
产品编号 ABIN7830610
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for E2F1 ELISA 试剂盒 (ABIN7830610)
抗原
See all E2F1 ELISA试剂盒
E2F1
(E2F Transcription Factor 1 (E2F1))
适用
人
检测方法
Colorimetric
实验类型
Cell ELISA
应用范围
ELISA
样品类型
Cell Samples, Suspension Cell Culture
-
-
原理
- E2F1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of E2F1 expression in cultured cells.
-
Analytical Method
- Qualitative
-
交叉反应
- 小鼠
-
-
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
板类型
- Uncoated
-
实验流程
-
- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C
-
储存方法
- Store the kit at 4°C.
-
-
- E2F1 (E2F Transcription Factor 1 (E2F1))
-
别名
- E2F1
-
背景
-
Synonyms: E2F-1|RBAP1|RBBP3|RBP3
Gene Description: E2F transcription factor 1
-
基因ID
- 1869
-
UniProt
- Q01094
-
途径
- p53 Pathway, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy
抗原 See all E2F1 ELISA试剂盒
-
You are here:
