CHEK2 ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for CHEK2 ELISA 试剂盒 (ABIN7830498)
抗原
See all CHEK2 ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
-
-
原理
- CHEK2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CHEK2 expression in cultured cells.
-
Analytical Method
- Qualitative
-
交叉反应
- 小鼠, 大鼠
-
-
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
板类型
- Uncoated
-
实验流程
-
- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C
-
储存方法
- Store the kit at 4°C.
-
-
- CHEK2 (Checkpoint Kinase 2 (CHEK2))
-
别名
- CHEK2
-
背景
-
Synonyms: CDS1|CHK2|HuCds1|LFS2|PP1425|RAD53
Gene Description: CHK2 checkpoint homolog (S. pombe)
-
基因ID
- 11200
-
UniProt
- O96017
-
途径
- p53 Pathway, Apoptosis, Cell Division Cycle
抗原 See all CHEK2 ELISA试剂盒
-