AKT1 ELISA 试剂盒
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Quick Overview for AKT1 ELISA 试剂盒 (ABIN7830406)
抗原
See all AKT1 ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- AKT1/AKT3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of AKT1/AKT3 expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA Kit
AKT1 适用: 人, 小鼠, 大鼠 pSer473 Colorimetric Sandwich ELISA Cell Lysate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 适用: 人, 小鼠, 大鼠 pSer473 Colorimetric Sandwich ELISA Cell Lysate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 适用: 小鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
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别名
- AKT1
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背景
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Synonyms: AKT|MGC99656|PKB|PKB-ALPHA|PRKBA|RAC|RAC-ALPHA
Gene Description: v-akt murine thymoma viral oncogene homolog 1
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基因ID
- 207
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UniProt
- P31749
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途径
- PI3K-Akt Signaling, RTK signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect
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