AKT1 ELISA 试剂盒
Quick Overview for AKT1 ELISA 试剂盒 (ABIN7672471)
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See all AKT1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
质量等级
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最低检测浓度
- 0.156 ng/mL
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原理
- Rat AKT1 (RAC-alpha serine/threonine-protein kinase) QuickTest ELISA Kit
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品牌
- QuickTest
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Analytical Method
- Quantitative
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灵敏度
- 0.094 ng/mL
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组件
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- ELISA Microplate (Dismountable)
- Lyophilized Standard
- Capture/Detection Antibody
- HRP-Streptavidin (orange)
- TMB Substrate
- Sample Dilution Buffer (blue)
- Stop Solution
- Wash Buffer (25x)
- Plate Sealer
- Product Description
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试剂未包括
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- Microplate reader (wavelength: 450nm)
- 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5 mL pipettor (for manual washing purpose)
- Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips (Calibration is required before use.)
- Sterile tubes and sample tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
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V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA Kit
AKT1 适用: 人, 小鼠, 大鼠 pSer473 Colorimetric Sandwich ELISA Cell Lysate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 适用: 人, 小鼠, 大鼠 pSer473 Colorimetric Sandwich ELISA Cell Lysate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 适用: 小鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) ELISA KitAKT1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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说明
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Traditional sandwich ELISA assay usually takes 3-4 hours. Most detection reagents in the market are concentrated and can't be distinguished from the colour. Dilution is required before use. We developed QuickTest ELISA kits to decrease customers’ operational errors, improve user's experience and get accurate assay result more efficiently. Compared with regular ELISA kits, QuickTest ELISA kits have more advantages in the same linearity, stability, recovery and storage conditions.
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样本量
- 50 µL
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实验时间
- 2 h
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板类型
- Pre-coated
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实验流程
- Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody pre-coated on the QuickTest plate. Add the Cap/Det Ab working solution into each well, then add the standards and pilot samples into relevant wells. Mix thoroughly and then incubate. If the sample contains detection target, a capture antibody-detection target-biotin-detection antibody complex was formed. After washing off unbound conjugates, HRP-Streptavidin was added to incubate. Repeat the washing process and then TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm with a microplate reader. The concentration of the detection target in the sample was calculated by plotting a standard curve. The concentration of the target substance is proportional to the OD450 value.
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实验流程
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- Take out the required strips, add 50 µL Cap/Det Ab into each well, then add 50 µL Standard or Sample into relevant well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure complete mixing then statically incubate for 60 minutes at 37 °C.
- Wash the plate twice without immersion.
- Add 100 µL HRP-Streptavidin (orange) into each well, seal the plate and statically incubate for 30 minutes at 37 °C.
- Wash the plate five times without immersion.
- Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C (Accurate TMB visualization control is required.).
- Add 50 µL stop solution. Read at 450nm immediately and calculate.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- 2-8 °C for 12 months
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有效期
- 12 months
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- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
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别名
- AKT1
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背景
- RAC-alpha serine/threonine-protein kinase|Protein kinase B|PKB|Protein kinase B alpha|PKB alpha|Proto-oncogene c-Akt|RAC-PK-alpha|AKT1|PKB|RAC
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UniProt
- P47196
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途径
- PI3K-Akt Signaling, RTK signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect
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