TGFB1 ELISA 试剂盒
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Quick Overview for TGFB1 ELISA 试剂盒 (ABIN7672379)
抗原
See all TGFB1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
质量等级
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最低检测浓度
- 31.25 pg/mL
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原理
- Human TGF-β1 (Transforming Growth Factor Beta 1) QuickTest ELISA Kit
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Analytical Method
- Quantitative
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特异性
- Specifically binds with TGF-β1 , no obvious cross reaction with other analogues. Recommended Sample Dilution Ratio The following table shows the recommended dilution ratios for this kit for a limited number of samples for your reference only. (When the sample content is very low, the original solution can be added without dilution, but it is necessary to ensure that the pH is between 6.8 and 8.0, and it does not contain more than 10 % organic solvents or high-concentration protein denaturants.) 1. Common sample validation: Sample Type Recommended Dilution Ratio Content Healthy serum (n=25) 1/50-1/100 dilution 30.5-92 ng/mL Healthy plasma (EDTA,heparin) (n=25) 1/5-1/10 dilution 1.4-6.8 ng/mL Healthy Urine (n=8) undiluted ND-95pg/mL Human peripheral blood mononuclear cells were cultured with 5 %FBS + 1640 + double antibody, and the cell culture supernatant was detected. 1/2 dilution 2.2 ng/mL Hela cell were cultured with 5 %FBS + 1640 + double antibody, and the cell culture supernatant was detected. 1/2 dilution 3.7 ng/mL 2. KO sample validation (Detect TGF-β1 KO A549 cells): Sample Type Dilution Ratio Content Wild A549 cells were cultured with 10 %FBS + 1640 + double antibody, and the cell culture supernatant was detected. 1/2 dilution 1.8 ng/mL Wild A549 cells were cultured with 10 %FBS + 1640 + double antibody and then cultured with 300 ng/mL Brefeldin A (BFA) for 3 hours. Add cell lysis buffer(Catalogue No.:E050), collect the lysate solution (total protein concentration measured by BCA assay: 2.3 mg/mL) to detect. 1/2 dilution 3.0 ng/mg(total protein) KO A549 cells were cultured with 10 %FBS + 1640 + double antibody and then cultured with 300 ng/mL Brefeldin A (BFA) for 3 hours. Add cell lysis buffer(Catalogue No.:E050), collect the lysate solution (total protein concentration measured by BCA assay: 1.9 mg/mL) to detect. undiluted ND Note:ND is lower than the sensitivity of the kit and was not detected 3. Capture antibody and detection antibody by WB KO validation (Detect TGF-β1 KO A549 cells):
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灵敏度
- 18.75 pg/mL
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试剂未包括
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- Microplate reader (wavelength: 450nm)
- 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5 mL pipettor
- Precision single (0.5-10μL
- 5-50μL
- 20-200μL
- 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
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Transforming Growth Factor, beta 1 (TGFB1) ELISA Kit
TGFB1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Platelet-Poor Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 人 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma, Serum
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Platelet-Poor Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Platelet-Poor Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 人 AA 279-390 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum, Urine
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 小鼠 AA 279-390 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum, Urine
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Platelet-Poor Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 小鸡 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 马 Colorimetric Competition ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum
Transforming Growth Factor, beta 1 (TGFB1) ELISA KitTGFB1 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Sandwich ELISA, Double Antibody
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样本量
- 50 μL
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实验时间
- 2 h
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板类型
- Pre-coated
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实验流程
- Step 1: Take out the required plate wells, add 50 µL Cap/Det Ab into each well, then add 50 µL Standard or Sample into individual well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure thorough mixing then static incubate for 60 minutes at 37 °C. Washing: Wash the plate twice without immersion. Step 2: Add 100 µL HRP-Streptavidin (orange) into each well, seal the plate and static incubate for 30 minutes at 37 °C. Washing: Wash the plate five times without immersion. Step 3: Add 90 µL TMB substrate solution, seal the plate and static incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 4: Add 50 µL stop solution. Read at 450nm immediately and calculate.
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实验流程
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- Take out the required strips, add 50 µL Cap/Det Ab into each well, then add 50 µL Standard or Sample into relevant well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure complete mixing then statically incubate for 60 minutes at 37 °C.
- Wash the plate twice without immersion.
- Add 100 µL HRP-Streptavidin (orange) into each well, seal the plate and statically incubate for 30 minutes at 37 °C.
- Wash the plate five times without immersion.
- Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C (Accurate TMB visualization control is required.).
- Add 50 µL stop solution. Read at 450nm immediately and calculate.
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限制
- 仅限研究用
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注意事项
- Avoid direct light exposure to antibody solutions
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储存条件
- 4 °C
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储存方法
- Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant
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有效期
- 12 months
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- TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))
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别名
- TGF-beta1
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背景
- Transforming growth factor beta-1 proprotein, Latency-associated peptide, LAP, Transforming growth factor beta-1, TGF-beta-1, TGFB1, TGFB
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UniProt
- P01137
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途径
- EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints
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