ACE2 ELISA 试剂盒
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Quick Overview for ACE2 ELISA 试剂盒 (ABIN6952471)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.025 ng/mL
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原理
- Human ACE-2 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair detects Human ACE-2. Other species not determined.
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产品特性
- Human ACE-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water/li>
- Precision pipettes to deliver 2 μL to 1 μL volumes/li>
- Adjustable 1-25 μL pipettes for reagent preparation/li>
- 100 μL and 1 liter graduated cylinders/li>
- Tubes to prepare standard and sample dilutions/li>
- Absorbent paper/li>
- Microplate reader capable of measuring absorbance at 450nm/li>
- Log-log graph paper or computer and software for ELISA data analysis
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Angiotensin I Converting Enzyme 2 (ACE2) ELISA Kit
ACE2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 人 Colorimetric Sandwich ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 人 Colorimetric Sandwich ELISA 0.39 ng/mL - 25 ng/mL Cell Culture Supernatant, Plasma, Serum
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 小鼠 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 小鼠 AA 18-740 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 人 Colorimetric Sandwich ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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样品制备
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Recommended Dilution (serum & plasma):8 fold
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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- ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))
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别名
- ACE-2
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途径
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
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