ACE2 ELISA 试剂盒
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Quick Overview for ACE2 ELISA 试剂盒 (ABIN1889360)
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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原理
- Sandwich Mouse ACE2 ELISA Kit to quantitate Mouse Ace2 in cell culture supernatants, serum and plasma (heparin).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: CHO
Immunogen sequence: Q18-T740Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Angiotensin I Converting Enzyme 2 (ACE2) ELISA Kit
ACE2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 人 Colorimetric Sandwich ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 小鼠 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 人 Colorimetric Sandwich ELISA 0.39 ng/mL - 25 ng/mL Cell Culture Supernatant, Plasma, Serum
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 大鼠 Colorimetric Sandwich ELISA 0.625-40 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 7.8%, 4.7%, 6.1%
Inter-Assay Precision (CV%): 8%, 5.7%, 7.1% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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: "Aliskiren Improves Ischemia- and Oxygen Glucose Deprivation-Induced Cardiac Injury through Activation of Autophagy and AMP-Activated Protein Kinase." in: Frontiers in pharmacology, Vol. 8, pp. 819, (2017) (PubMed).
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- ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))
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别名
- ACE2
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背景
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Background: AngiotensinI-converting enzyme 2 (ACE 2) is a protein belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. By sequence similarity to a sequence in GenBank, this gene is mapped to Xp22.2. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.
Gene Full Name: angiotensin converting enzyme 2
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基因ID
- 70008
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UniProt
- Q8R0I0
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途径
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
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