ACE2 ELISA 试剂盒
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Quick Overview for ACE2 ELISA 试剂盒 (ABIN6720239)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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Supplier Product No.
- koa0687
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Supplier
- Rockland
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原理
- Mouse ACE2 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: CHO
Immunogen sequence: Q18-T740 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Angiotensin I Converting Enzyme 2 (ACE2) ELISA Kit
ACE2 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 小鼠 AA 18-740 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 人 Colorimetric Sandwich ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 小鼠 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitQuick ACE2 适用: 人 Colorimetric Sandwich ELISA 0.39 ng/mL - 25 ng/mL Biological Fluids, Plasma, Serum
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitQuick ACE2 适用: 大鼠 Colorimetric Sandwich ELISA 0.39 ng/mL - 25 ng/mL Biological Fluids, Plasma, Serum
Angiotensin I Converting Enzyme 2 (ACE2) ELISA KitACE2 适用: 人 Colorimetric Sandwich ELISA 0.39 ng/mL - 25 ng/mL Cell Culture Supernatant, Plasma, Serum
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说明
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Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse ACE2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse ACE2 standard solution and each sample is measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 7.8%, 4.7%, 6.1%
Inter-Assay Precision (CV%): 8%, 5.7%, 7.1% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))
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别名
- ACE2
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背景
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Background: AngiotensinI-converting enzyme 2 (ACE 2) is a protein belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. By sequence similarity to a sequence in GenBank, this gene is mapped to Xp22.2. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.
Gene Full Name: angiotensin converting enzyme 2
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基因ID
- 70008
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NCBI登录号
- NP_001123985
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UniProt
- Q8R0I0
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途径
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
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