ATG4A ELISA 试剂盒
This 人 ATG4A ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 ATG4A in Cell Culture Supernatant, Cell Samples, Plasma, Serum 和 Tissue Lysate.
Quick Overview for ATG4A ELISA 试剂盒 (ABIN6951537)
抗原
See all ATG4A products
ATG4A
(Autophagy related 4A Cysteine Peptidase (ATG4A))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
样品类型
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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原理
- Human ATG4A ELISA Kit.
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Analytical Method
- Quantitative
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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有效期
- 6 months
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- ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))
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别名
- ATG4A
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基因ID
- 115201
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UniProt
- Q8WYN0
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途径
- Autophagy
抗原 See all ATG4A products
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