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CTLA4 ELISA 试剂盒

Name: CTLA4 ELISA 试剂盒
SKU: ABIN6951398
Availability: OutOfStock

This 大鼠 CTLA4 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 大鼠 CTLA4.

产品编号 ABIN6951398

发货至: 中国

Quick Overview for CTLA4 ELISA 试剂盒 (ABIN6951398)

抗原

See all CTLA4 ELISA试剂盒

CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

适用

大鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

应用范围

ELISA

样品类型

Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Discover our top product CTLA4 ELISA Kit

原理

Rat CTLA-4 ELISA Kit.

Analytical Method

Quantitative

产品特性
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
组件
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
试剂未包括
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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CTLA4 适用: 山羊 Colorimetric Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

应用备注

Optimal working dilution should be determined by the investigator.

板类型

Pre-coated

实验流程
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.
限制

仅限研究用
有效期

6 months
抗原 See all CTLA4 ELISA试剂盒

CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

别名

CTLA-4

基因ID

63835

UniProt

Q62859

途径

Cancer Immune Checkpoints