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CTLA4 ELISA 试剂盒
This 大鼠 CTLA4 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 大鼠 CTLA4 in Cell Culture Supernatant, Cell Samples, Plasma, Serum 和 Tissue Lysate.
Quick Overview for CTLA4 ELISA 试剂盒 (ABIN6951398)
抗原
See all CTLA4 ELISA试剂盒
CTLA4
(Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))
适用
All reactivities for CTLA4 ELISA试剂盒
大鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
样品类型
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
CTLA4ELISA Kit详情
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原理
Rat CTLA-4 ELISA Kit.
Analytical Method
Quantitative
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternatives
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使用细节
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应用备注
Optimal working dilution should be determined by the investigator.
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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有效期
6 months
CTLA4目标详情
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CTLA4
(Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))
别名
CTLA-4
基因ID
63835
UniProt
Q62859
途径
Cancer Immune Checkpoints
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