CTLA4 ELISA 试剂盒
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Quick Overview for CTLA4 ELISA 试剂盒 (ABIN3199209)
抗原
See all CTLA4 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 93.75 pg/mL
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原理
- This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human CTLA-4.
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Analytical Method
- Quantitative
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特异性
- CTLA-4/CD152 ELISA Kit, human
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组件
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- Plate
- Standard
- Diluent
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试剂未包括
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Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.
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Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) ELISA Kit
CTLA4 适用: 小鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) ELISA KitCTLA4 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) ELISA KitCTLA4 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) ELISA KitCTLA4 适用: 小鼠 AA 37-162 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) ELISA KitCTLA4 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) ELISA KitCTLA4 适用: 小鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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实验时间
- 3 - 4 h
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- protect your eyes
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储存条件
- 4 °C
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储存方法
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Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit
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- CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))
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别名
- CTLA-4/CD152
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背景
- Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), also known as CD152, is a member of the immunoglobulin superfamily that is expressed on the surface of helper T cells. CTLA-4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86.
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基因ID
- 1493
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NCBI登录号
- NM_005214
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UniProt
- P16410
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途径
- Cancer Immune Checkpoints
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