Phospholipase C gamma 1 ELISA 试剂盒
Quick Overview for Phospholipase C gamma 1 ELISA 试剂盒 (ABIN6730785)
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See all Phospholipase C gamma 1 (PLCG1) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- Human, Mouse and Rat Phospho-PLCG1 (Tyr771) ELISA Kit. This assay semi-quantitatively measures phosphorylated PLCG1 (Tyr771) in lysate samples.
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Analytical Method
- Semi-Quantitative
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特异性
- This ELISA kit recognizes Human, Mouse, and Rat PLCG1 (Tyr771)
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产品特性
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- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
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试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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phospholipase C, gamma 1 (PLCG1) ELISA Kit
PLCG1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
phospholipase C, gamma 1 (PLCG1) ELISA KitPLCG1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
phospholipase C, gamma 1 (PLCG1) ELISA KitPLCG1 适用: 大鼠 Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
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有效期
- 6 months
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- Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))
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别名
- PLCG1
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基因ID
- 5335
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途径
- RTK signaling, WNT signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling
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