Caspase 3 ELISA 试剂盒
Quick Overview for Caspase 3 ELISA 试剂盒 (ABIN6720394)
抗原
See all Caspase 3 (CASP3) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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Supplier Product No.
- koa0860
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Supplier
- Rockland
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原理
- Human Caspase 3 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human Caspase 3. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Caspase 3 (CASP3) ELISA Kit
CASP3 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Caspase 3 (CASP3) ELISA KitCASP3 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
SensoLyte® Homogeneous AMC Caspase-3/7 Assay KitFRET Fluorometric
Caspase 3 (CASP3) ELISA KitCASP3 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Caspase 3 (CASP3) ELISA KitCASP3 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Caspase 3 (CASP3) ELISA KitCASP3 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Caspase 3 (CASP3) ELISA KitCASP3 适用: Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Caspase 3 (CASP3) ELISA KitCASP3 适用: 小鼠 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Caspase 3 (CASP3) ELISA KitCASP3 适用: 犬 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Caspase 3 (CASP3) ELISA KitCASP3 适用: 兔 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Caspase 3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, tissue homogenates or serum to each empty well. It is recommended that each human Caspase 3 standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: S29-D175&A183-H277
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Caspase 3 (CASP3)
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别名
- Caspase 3
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背景
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Synonyms: CASP-3, CASP3, CASP 3, Caspase-3, Caspase3, Caspase 3, SCA-1, SCA 1, SCA1
Background: Caspase-3 is a caspase protein that is encoded by the CASP3 gene. This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
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基因ID
- 836
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NCBI登录号
- NP_004337
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UniProt
- P42574
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途径
- Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
抗原 See all Caspase 3 (CASP3) ELISA试剂盒
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