CXCL14 ELISA 试剂盒
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北京 101111
Quick Overview for CXCL14 ELISA 试剂盒 (ABIN6720223)
抗原
See all CXCL14 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 125 pg/mL
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Supplier Product No.
- koa0761
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Supplier
- Rockland
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原理
- Human CXCL14 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: S35-E111 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 60 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA Kit
CXCL14 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA KitCXCL14 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA KitCXCL14 适用: 大鼠 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA KitCXCL14 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA KitCXCL14 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA KitCXCL14 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) CLIA KitCXCL14 适用: 人 Chemiluminescent Sandwich ELISA 31.25 pg/mL - 2000 pg/mL
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA KitCXCL14 适用: Hamster AA 35-111 Colorimetric Sandwich ELISA 125 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA KitCXCL14 适用: Pig AA 35-111 Colorimetric Sandwich ELISA 125 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA KitCXCL14 适用: 马 AA 35-111 Colorimetric Sandwich ELISA 125 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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说明
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Capture antibody and Detection antibody: monoclonal antibody from mouse, monoclonal antibody from mouse
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CXCL14 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CXCL14 standard solution and each sample be measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 4.2%, 7.5%, 7.1%
Inter-Assay Precision (CV%): 5.5%, 8.9%, 7.4% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))
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别名
- CXCL14
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背景
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Background: Chemokine (C-X-C motif) ligand 14 (CXCL14) is a small cytokine belonging to the CXC chemokine family that is also known as BRAK (for breast and kidney-expressed chemokine). This gene is mapped to 5q31.1. CXCL14 is constitutively expressed at high levels in many normal tissues, it has been found that fibroblasts are the primary source of CXCL14 and that CXCL14 is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation. It is also a potent chemoattractant and activator of dendritic cells, is implicated in homing of these cells, and can stimulate the migration of activated NK cells. CXCL14 also inhibits angiogenesis, possibly as a result of its ability to block endothelial cell chemotaxis. In addition to it, CXCL14 is a potent chemokine for immature DCs, with a chemoattractive activity comparable to bacterial fMLP and MIP1A.
Gene Full Name: C-X-C motif chemokine ligand 14
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基因ID
- 9547
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NCBI登录号
- NP_004878
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UniProt
- O95715
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途径
- Autophagy
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