CXCL14 ELISA 试剂盒
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北京 101111
Quick Overview for CXCL14 ELISA 试剂盒 (ABIN1889427)
抗原
See all CXCL14 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 125 pg/mL
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原理
- Sandwich Human CXCL14/Brak ELISA Kit to quantitate Human CXCL14 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: S35-E111Capture antibody and Detection antibody: monoclonal antibody from mouse, monoclonal antibody from mouse
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 60 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA Kit
CXCL14 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA KitCXCL14 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA KitCXCL14 适用: 大鼠 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA KitCXCL14 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA KitCXCL14 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA KitCXCL14 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) CLIA KitCXCL14 适用: 人 Chemiluminescent Sandwich ELISA 31.25 pg/mL - 2000 pg/mL
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA KitCXCL14 适用: Cow AA 35-111 Colorimetric Sandwich ELISA 125 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA KitCXCL14 适用: Pig AA 35-111 Colorimetric Sandwich ELISA 125 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-X-C Motif) Ligand 14 (CXCL14) ELISA KitCXCL14 适用: Hamster AA 35-111 Colorimetric Sandwich ELISA 125 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.2%, 7.5%, 7.1%
Inter-Assay Precision (CV%): 5.5%, 8.9%, 7.4% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))
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别名
- CXCL14
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背景
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Background: Chemokine (C-X-C motif) ligand 14 (CXCL14) is a small cytokine belonging to the CXC chemokine family that is also known as BRAK (for breast and kidney-expressed chemokine). This gene is mapped to 5q31.1. CXCL14 is constitutively expressed at high levels in many normal tissues, it has been found that fibroblasts are the primary source of CXCL14 and that CXCL14 is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation. It is also a potent chemoattractant and activator of dendritic cells, is implicated in homing of these cells, and can stimulate the migration of activated NK cells. CXCL14 also inhibits angiogenesis, possibly as a result of its ability to block endothelial cell chemotaxis. In addition to it, CXCL14 is a potent chemokine for immature DCs, with a chemoattractive activity comparable to bacterial fMLP and MIP1A.
Gene Full Name: C-X-C motif chemokine ligand 14
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基因ID
- 9547
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UniProt
- O95715
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途径
- Autophagy
抗原 See all CXCL14 ELISA试剂盒
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