DIABLO ELISA 试剂盒
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- 抗原 See all DIABLO ELISA试剂盒
- DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2 pg/mL - 2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich ELISA for Quantitative Detection of Antigen
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 产品特性
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Synonyms: DFNA64, DIABLO, diablo homolog, mitochondrial, diablo, IAP-binding mitochondrial protein, DIABLO-S, direct IAP-binding protein with low pI, mitochondrial Smac protein, second mitochondria-derived activator of caspase, SMAC3
Background: Diablo homolog, mitochondrial, also known as second mitochondria-derived activator of caspases(SMAC), is a protein that in humans is encoded by the DIABLO gene(direct IAP binding protein with low pI). It is mapped to 12q24.31. DIABLO can bind mammalian IAP homolog A(MIHA, or API3) and can also interact with MIHB, MIHC, and OpIAP, the baculoviral IAP. This gene encodes an inhibitor of apoptosis protein(IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Over expression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64.
Gene Name: DIABLO
Production: Natural and recombinant human Diablo. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: E.coli, Immunogen sequence: A56-D239
- Top Product
- Discover our top product DIABLO ELISA Kit
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Second Mitochondria-Derived Activator of Caspase (DIABLO) ELISA Kit
DIABLO 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Second Mitochondria-Derived Activator of Caspase (DIABLO) ELISA KitDIABLO 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Second Mitochondria-Derived Activator of Caspase (DIABLO) ELISA KitDIABLO 适用: 人 Colorimetric Sandwich ELISA 0.1-30 ng/mL Cell Culture Supernatant, Plasma, Serum
Second Mitochondria-Derived Activator of Caspase (DIABLO) ELISA KitDIABLO 适用: 人 AA 56-239 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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- 应用备注
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Diablo standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Diablo standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 31.2pg/mL-2000pg/mL
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
-
- 抗原 See all DIABLO ELISA试剂盒
- DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))
- 别名
- Diablo - SMAC (DIABLO 产品)
- 别名
- smac ELISA Kit, smac3 ELISA Kit, diablo-s ELISA Kit, 0610041G12Rik ELISA Kit, 1700006L01Rik ELISA Kit, AU040403 ELISA Kit, Smac ELISA Kit, DFNA64 ELISA Kit, SMAC ELISA Kit, DBO ELISA Kit, XSmac ELISA Kit, diablo, IAP-binding mitochondrial protein ELISA Kit, diablo IAP-binding mitochondrial protein ELISA Kit, diablo, IAP-binding mitochondrial protein L homeolog ELISA Kit, diablo ELISA Kit, Diablo ELISA Kit, DIABLO ELISA Kit, diablo.L ELISA Kit
- 基因ID
- 56616
- NCBI登录号
- NP_001265231
- UniProt
- Q9NR28
- 途径
- Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
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