DIABLO ELISA 试剂盒
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- DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))
- 抗原表位
- AA 56-239
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Diablo/SMAC
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: A56-D239 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: A56-D239 - Featured
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Second Mitochondria-Derived Activator of Caspase (DIABLO) ELISA Kit
DIABLO 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Second Mitochondria-Derived Activator of Caspase (DIABLO) ELISA KitDIABLO 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Second Mitochondria-Derived Activator of Caspase (DIABLO) ELISA KitDIABLO 适用: 人 Colorimetric Sandwich ELISA 0.1-30 ng/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed. .
- 板类型
- Pre-coated
- 实验流程
- human Diablo ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Diablo has been precoated onto 96-well plates. Standards(E.coli, A56-D239) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Diablo is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Diablo amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Diablo standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Diablo standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 112, Standard deviation: 6.27, CV(%): 5.6
- Sample 2: n=16, Mean(pg/ml): 545, Standard deviation: 23.98, CV(%): 4.4
- Sample 3: n=16, Mean(pg/ml): 1134, Standard deviation: 58.97, CV(%): 5.2,
- Sample 1: n=24, Mean(pg/ml): 129, Standard deviation: 7.99, CV(%): 6.2
- Sample 2: n=24, Mean(pg/ml): 577, Standard deviation: 40.97, CV(%): 7.1
- Sample 3: n=24, Mean(pg/ml): 1133, Standard deviation: 77.04, CV(%): 6.8
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all DIABLO ELISA试剂盒
- DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))
- 别名
- DIABLO (DIABLO 产品)
- 别名
- smac ELISA Kit, smac3 ELISA Kit, diablo-s ELISA Kit, 0610041G12Rik ELISA Kit, 1700006L01Rik ELISA Kit, AU040403 ELISA Kit, Smac ELISA Kit, DFNA64 ELISA Kit, SMAC ELISA Kit, DBO ELISA Kit, XSmac ELISA Kit, diablo, IAP-binding mitochondrial protein ELISA Kit, diablo IAP-binding mitochondrial protein ELISA Kit, diablo, IAP-binding mitochondrial protein L homeolog ELISA Kit, diablo ELISA Kit, Diablo ELISA Kit, DIABLO ELISA Kit, diablo.L ELISA Kit
- 背景
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Protein Function: Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis- inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance. .
Background: Diablo homolog, mitochondrial, also known as second mitochondria-derived activator of caspases(SMAC), is a protein that in humans is encoded by the DIABLO gene(direct IAP binding protein with low pI). It is mapped to 12q24.31. DIABLO can bind mammalian IAP homolog A(MIHA, or API3) and can also interact with MIHB, MIHC, and OpIAP, the baculoviral IAP. This gene encodes an inhibitor of apoptosis protein(IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Over expression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64.
Synonyms: Diablo homolog, mitochondrial,Direct IAP-binding protein with low pI,Second mitochondria-derived activator of caspase,Smac,DIABLO,SMAC,
Full Gene Name: Diablo homolog, mitochondrial
Cellular Localisation: Mitochondrion . Released into the cytosol when cells undergo apoptosis. - 基因ID
- 56616
- UniProt
- Q9NR28
- 途径
- Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
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