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IGF1R ELISA 试剂盒

This 人 IGF1R ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 IGF1R.
Rockland
产品编号 ABIN6720107
Supplier Product No.: koa0457
发货至: 中国

Quick Overview for IGF1R ELISA 试剂盒 (ABIN6720107)

抗原

See all IGF1R ELISA试剂盒
IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

适用

  • 10
  • 4
  • 4
  • 1
  • 1

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

156 pg/mL - 10000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    156 pg/mL

    Supplier Product No.

    koa0457

    Supplier

    Rockland

    原理

    Human IGF1R Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant human IGF1R. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human IGF1R standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IGF1R standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: E31-N932

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all IGF1R ELISA试剂盒

    IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

    别名

    IGF1R

    背景

    Synonyms: CD221, IGF 1R, IGF I receptor, IGF-I receptor, Igf1r, IGF1R_HUMAN, IGFIR, IGFR, Insulin like growth factor 1 receptor, Insulin like growth factor I receptor, Insulin-like growth factor 1 receptor beta chain, Insulin-like growth factor I receptor, JTK13, MGC142170, MGC142172, MGC18216

    Background: IGF1R, also called IGFR or insulin-like growth factor 1 receptor, is a protein that found on the surface of human cells. This gene is mapped to 15q26.3. IGF1R belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in growth and continues to have anabolic effects in adults - meaning that it can induce hypertrophy of skeletal muscle and other target tissues. IGF1R also plays a critical role in transformation events. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.

    基因ID

    3480

    NCBI登录号

    NP_000866

    UniProt

    P08069

    途径

    RTK signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy
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