CD40 ELISA 试剂盒
Quick Overview for CD40 ELISA 试剂盒 (ABIN6720056)
抗原
See all CD40 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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Supplier Product No.
- koa0408
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Supplier
- Rockland
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原理
- Mouse CD40 - TNFRSF5 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant mouse CD40. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 1 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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CD40 (CD40) ELISA Kit
CD40 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
CD40 (CD40) ELISA KitCD40 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD40 (CD40) ELISA KitCD40 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
CD40 (CD40) ELISA KitCD40 适用: 人 Colorimetric Sandwich ELISA 50-40000 pg/mL Cell Culture Supernatant, Plasma, Serum
CD40 (CD40) ELISA KitCD40 适用: 小鼠 Colorimetric Sandwich ELISA 3-1500 pg/mL Cell Culture Supernatant, Plasma, Serum
CD40 (CD40) ELISA KitCD40 适用: 人 Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
CD40 (CD40) ELISA KitCD40 适用: 人 AA 21-193 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
CD40 (CD40) ELISA KitCD40 适用: 小鼠 AA 24-193 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
CD40 (CD40) ELISA KitCD40 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Urine
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse CD40 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, cell lysates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CD40 standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: V24-R193
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- CD40
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别名
- CD40/TNFRSF5
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背景
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Synonyms: B cell associated molecule|B cell associated molecule CD40, B cell surface antigen CD40, Bp50, CD40 antigen, CD40 Molecule, CD40 Molecule TNF receptor superfamily member 5, CD40 protein, CD40 type II isoform, CD40L receptor, Nerve growth factor receptor related B lymphocyte activation molecule, NFRSF5, p50, T BAM, TBAM, TNF receptor superfamily member 5, TNFRSF5, TRAP, Tumor necrosis factor receptor superfamily member 5, Tumor necrosis factor receptor superfamily member 5 precursor
Background: CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
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基因ID
- 21939
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NCBI登录号
- NP_035741
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UniProt
- P27512
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途径
- NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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