TNFSF8 ELISA 试剂盒
Quick Overview for TNFSF8 ELISA 试剂盒 (ABIN6720055)
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See all TNFSF8 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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Supplier Product No.
- koa0348
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Supplier
- Rockland
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原理
- Mouse CD30L Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant mouse CD30L. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 6 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) ELISA Kit
TNFSF8 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) ELISA KitTNFSF8 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) ELISA KitTNFSF8 适用: 大鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) ELISA KitTNFSF8 适用: 小鼠 AA 68-239 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) ELISA KitTNFSF8 适用: 犬 Colorimetric Competition ELISA 0.5-10 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) ELISA KitTNFSF8 适用: 小鼠 Colorimetric Sandwich ELISA 0.5-100 pg/mL Cell Culture Supernatant, Plasma, Serum
Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8) ELISA KitTNFSF8 适用: 小鸡 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL mouse CD30L standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse CD30L standard solution and each sample is measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: Q68-D239
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))
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别名
- CD30L
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背景
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Synonyms: CD153 antigen, CD30 antigen ligand, CD30 L, CD30 ligand, CD30L, CD30LG, MGC138144, TNFSF8, Tumor necrosis factor (ligand) superfamily member 8, Tumor necrosis factor ligand superfamily member 8
Background: The human CD30L(CD153) gene is located at 9q33. It contains 4 exons and spans more than 17.1 kb. CD153 is expressed on the surface of B cells and this expression is upregulated upon CD154(CD40LG), IL4, and B-cell receptor engagement. In these cells, engagement of CD153 by T cell CD30 inhibits immunoglobulin class switch recognition as well as IgG, IgA, and IgE production, suggesting that this "reverse signaling" modulates the CD154-dependent switching of B cells into the pool producing IgG, IgA, and IgE. Additionally, recombinant human CD30L enhanced the proliferation of CD3 -activated T cells, but induced differential responses, including cell death, in several CD30-positive lymphoma-derived cell lines. The standard product used in this kit is recombinant mouse CD30L with the molecular mass of 30-45KDa.
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基因ID
- 21949
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NCBI登录号
- NP_033429
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UniProt
- P32972
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