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Chemerin ELISA 试剂盒

This 小鼠 Chemerin ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 Chemerin.
Rockland
产品编号 ABIN6720015
Supplier Product No.: koa0799
发货至: 中国

Quick Overview for Chemerin ELISA 试剂盒 (ABIN6720015)

抗原

See all Chemerin (RARRES2) ELISA试剂盒
Chemerin (RARRES2) (Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2))

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

0.78 ng/mL - 50 ng/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    0.78 ng/mL

    Supplier Product No.

    koa0799

    Supplier

    Rockland

    原理

    Mouse Chemerin - RARRES2 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant mouse Chemerin. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 20 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse Chemerin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse Chemerin standard solution and each sample is measured in duplicate.

    说明

    Standard: Expression system for standard: E.coli, Immunogen sequence: T20-K162

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all Chemerin (RARRES2) ELISA试剂盒

    Chemerin (RARRES2) (Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2))

    别名

    Chemerin/RARRES2

    背景

    Synonyms: chemerin, HP10433, RAR-responsive protein TIG2, RARRES2, retinoic acid receptor responder (tazarotene induced) 2, retinoic acid receptor responder protein 2, tazarotene-induced gene 2 protein, TIG3

    Background: Chemerin, also known as RARRES2 or TIG2, is a protein that is encoded by the RARRES2 gene. It is mapped to 7q36.1. Chemerin is a potent chemoattractant specific for antigen-presenting cells that requires proteolytic activation and acts as a ligand for the G protein-coupled receptor CMKLR1(also known as ChemR23). It is a 14 kDa protein secreted in an inactive form as prochemerin and is activated through cleavage of the C-terminus by inflammatory and coagulation serine proteases. Chemerin was found to stimulate chemotaxis of dendritic cells and macrophages to the site of inflammation. What's more, the active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein.

    基因ID

    71660

    NCBI登录号

    NP_082128

    UniProt

    Q9DD06

    途径

    Brown Fat Cell Differentiation
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