Chemerin ELISA 试剂盒
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Quick Overview for Chemerin ELISA 试剂盒 (ABIN2859336)
抗原
See all Chemerin (RARRES2) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.78 ng/mL
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原理
- Sandwich Mouse Chemerin/RARRES2 ELISA Kit to quantitate Mouse Rarres2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: T20-K162Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 20 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA Kit
RARRES2 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA KitRARRES2 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA KitRARRES2 适用: 大鼠 Colorimetric Sandwich ELISA 0.9 ng/mL - 60 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA KitRARRES2 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA KitRARRES2 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA KitRARRES2 适用: 小鼠 Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA KitRARRES2 适用: Pig Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA KitRARRES2 适用: 小鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA KitRARRES2 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA KitRARRES2 适用: 人 AA 17-163 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 7.4%, 6.9%, 6.9%
Inter-Assay Precision (CV%): 7.4%, 7.4%, 8.3% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- Chemerin (RARRES2) (Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2))
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别名
- RARRES2
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背景
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Background: Chemerin, also known as RARRES2 or TIG2, is a protein that is encoded by the RARRES2 gene. It is mapped to 7q36.1. Chemerin is a potent chemoattractant specific for antigen-presenting cells that requires proteolytic activation and acts as a ligand for the G protein-coupled receptor CMKLR1 (also known as ChemR23). It is a 14 kDa protein secreted in an inactive form as prochemerin and is activated through cleavage of the C-terminus by inflammatory and coagulation serine proteases. Chemerin was found to stimulate chemotaxis of dendritic cells and macrophages to the site of inflammation. What's more, the active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein.
Gene Full Name: retinoic acid receptor responder (tazarotene induced) 2
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基因ID
- 71660
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UniProt
- Q9DD06
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途径
- Brown Fat Cell Differentiation
抗原 See all Chemerin (RARRES2) ELISA试剂盒
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