Chemerin ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for Chemerin ELISA 试剂盒 (ABIN6720010)
抗原
See all Chemerin (RARRES2) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 78 pg/mL
-
Supplier Product No.
- koa0798
-
Supplier
- Rockland
-
原理
- Human Chemerin - RARRES2 Sandwich ELISA Kit for Quantitative Detection
-
品牌
- AccuSignal™
-
Analytical Method
- Quantitative
-
特异性
-
Expression system for standard: E.coli
Immunogen sequence: V17-S163 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- < 20 pg/mL
-
组件
-
- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
-
试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
-
-
Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA Kit
RARRES2 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA KitRARRES2 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA KitRARRES2 适用: 大鼠 Colorimetric Sandwich ELISA 0.9 ng/mL - 60 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA KitRARRES2 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA KitRARRES2 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA KitRARRES2 适用: 小鼠 Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA KitRARRES2 适用: Pig Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA KitRARRES2 适用: 小鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA KitQuick RARRES2 适用: 小鼠 Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Biological Fluids, Plasma, Serum
Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2) ELISA KitQuick RARRES2 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Biological Fluids, Plasma, Serum
-
-
说明
-
Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Chemerin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human Chemerin standard solution and each sample is measured in duplicate.
-
实验精密度
-
Intra-Assay Precision (CV%): 4.7%, 6.4%, 7.6%
Inter-Assay Precision (CV%): 6%, 8.1%, 9.3% -
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
-
有效期
- 12 months
-
-
- Chemerin (RARRES2) (Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2))
-
别名
- Chemerin/RARRES2
-
背景
-
Background: Chemerin, also known as RARRES2 or TIG2, is a protein that in humans is encoded by the RARRES2 gene. It is mapped to 7q36.1. Chemerin is a potent chemoattractant specific for antigen-presenting cells that requires proteolytic activation and acts as a ligand for the G protein-coupled receptor CMKLR1 (also known as ChemR23). It is a 14 kDa protein secreted in an inactive form as prochemerin and is activated through cleavage of the C-terminus by inflammatory and coagulation serine proteases. Chemerin was found to stimulate chemotaxis of dendritic cells and macrophages to the site of inflammation. What's more, the active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein.
Gene Full Name: retinoic acid receptor responder 2
-
基因ID
- 5919
-
NCBI登录号
- NP_002880
-
UniProt
- Q99969
-
途径
- Brown Fat Cell Differentiation
抗原 See all Chemerin (RARRES2) ELISA试剂盒
-
