FLT3LG ELISA 试剂盒
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Quick Overview for FLT3LG ELISA 试剂盒 (ABIN6719986)
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See all FLT3LG ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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Supplier Product No.
- koa0172
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Supplier
- Rockland
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原理
- Human Flt-3ligand Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: T27-P185 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA Kit
FLT3LG 适用: 大鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: Pig Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: 小鼠 Colorimetric Sandwich ELISA 0.78 pg/mL - 50 pg/mL Plasma, Serum
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: 人 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: 小鼠 AA 27-188 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Serum
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: 人 Colorimetric Sandwich ELISA 16-1000 pg/mL Cell Lysate, Plasma, Serum
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: 大鼠 AA 27-188 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Serum
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说明
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Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human Flt-3ligand standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human Flt-3ligand standard solution and each sample be measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 7.6%, 6%, 7.8%
Inter-Assay Precision (CV%): 9.1%, 7.2%, 8.9% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))
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别名
- Flt-3ligand
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背景
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Background: Fms-related tyrosine kinase 3 ligand (FLT3LG), also known as Flt-3ligand or FL, is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). In synergy with other growth factors, Flt-3ligand stimulates the proliferation and differentiation of various blood cell progenitors. Besides that, Flt-3ligand can control the development of Dendritic cells (DCs) and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt-3ligand also can enhance the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells.
Gene Full Name: fms related receptor tyrosine kinase 3 ligand
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基因ID
- 2323
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NCBI登录号
- NP_001191431
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UniProt
- P49771
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途径
- RTK signaling
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