FLT3LG ELISA 试剂盒
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Quick Overview for FLT3LG ELISA 试剂盒 (ABIN1889292)
抗原
See all FLT3LG ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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原理
- Sandwich Mouse Flt-3 Ligand ELISA Kit to quantitate Mouse Flt3lg in cell culture supernatants and serum.
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: G27-R188Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA Kit
FLT3LG 适用: 大鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: Pig Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: 小鼠 Colorimetric Sandwich ELISA 0.78 pg/mL - 50 pg/mL Plasma, Serum
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: 人 Colorimetric Sandwich ELISA 16-1000 pg/mL Cell Lysate, Plasma, Serum
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: 人 AA 27-185 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: 人, 猕猴 Colorimetric Sandwich ELISA 12-400 pg/mL Cell Culture Supernatant, Plasma, Serum
Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) ELISA KitFLT3LG 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 5%, 4.3%, 6.7%
Inter-Assay Precision (CV%): 6.2%, 6%, 7.2% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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: "The role of RIP1 and RIP3 in the development of aplastic anemia induced by cyclophosphamide and busulphan in mice." in: International journal of clinical and experimental pathology, Vol. 7, Issue 12, pp. 8411-20, (2015) (PubMed).
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- FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))
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别名
- FLT3LG
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背景
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Background: Fms-related tyrosine kinase 3 ligand (FLT3LG), also known as Flt-3ligand or FL, is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). In synergy with other growth factors, Flt-3ligand stimulates the proliferation and differentiation of various blood cell progenitors. Besides that, Flt-3ligand can control the development of Dendritic cells (DCs) and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt-3ligand also can enhance the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells.
Gene Full Name: FMS-like tyrosine kinase 3 ligand
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基因ID
- 14256
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UniProt
- P49772
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途径
- RTK signaling
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