ADAMTS13 ELISA 试剂盒
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- 抗原 See all ADAMTS13 ELISA试剂盒
- ADAMTS13 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.78 ng/mL - 50 ng/mL
- 最低检测浓度
- 0.78 ng/mL
- 应用范围
- ELISA
- Supplier Product No.
- koa0563
- Supplier
- Rockland
- 原理
- Human ADAMTS13 Sandwich ELISA Kit for Quantitative Detection
- 品牌
- AccuSignal™
- 样品类型
- Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
- Analytical Method
- Quantitative
- 特异性
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Production: Natural and recombinant human ADAMTS13. There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- < 20 pg/mL
- 组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product ADAMTS13 ELISA Kit
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ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13) ELISA Kit
ADAMTS13 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13) ELISA KitADAMTS13 适用: 大鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13) ELISA KitADAMTS13 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13) ELISA KitADAMTS13 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13) ELISA KitADAMTS13 适用: 人 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13) ELISA KitADAMTS13 适用: 人 AA 34-688 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
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- 应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human ADAMTS13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. We recommend that each human ADAMTS13 standard solution and each sample is measured in duplicate.
- 说明
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Standard: Expression system for standard: CHO, Immunogen sequence: Q34-W688
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原 See all ADAMTS13 ELISA试剂盒
- ADAMTS13 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13))
- 别名
- ADAMTS13 (ADAMTS13 产品)
- 背景
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Synonyms: A disintegrin and metalloproteinase with thrombospondin motifs 13, A disintegrin like and metalloprotease with thrombospondin type 1 motif 13, A disintegrin-like and metalloprotease (Reprolysin type) with thrombospondin type 1 motif 13, ADAM metallopeptidase with thrombospondin type 1 motif 13, ADAM TS, ADAMTS 13, ADAMTS13, ADAMTS13 protein, ATS13_HUMAN, TTP, Von Willebrand factor cleaving protease, vWF cleaving protease, vWFCP
Background: ADAMTS13 is a zinc-containing metalloprotease enzyme. The ADAMTS13 gene is mapped to chromosome 9q34 by genomic sequence analysis. It is reported that a metal-containing proteolytic enzyme (metalloprotease) in normal plasma cleaves the peptide bond between tyrosine at position 842 and methionine at position 843 in monomeric subunits of von Willebrand factor, thereby degrading the large multimers. And it is confirmed that the ADAMTS13 gene encodes the von Willebrand factor-cleaving protease (VWFCP). ADAMTS13 is secreted in blood and degrades large vWf multimers, decreasing their activity.
- 基因ID
- 11093
- NCBI登录号
- NP_620594
- UniProt
- Q76LX8
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