CTLA4 ELISA 试剂盒
Quick Overview for CTLA4 ELISA 试剂盒 (ABIN6719944)
抗原
See all CTLA4 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0418
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Supplier
- Rockland
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原理
- Mouse CTLA4 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant mouse CTLA4. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) ELISA Kit
CTLA4 适用: 小鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) ELISA KitCTLA4 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) ELISA KitCTLA4 适用: 小鼠 AA 37-162 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) ELISA KitCTLA4 适用: 人 Colorimetric Sandwich ELISA 93.75-6000 pg/mL Cell Culture Supernatant, Plasma, Serum
Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) ELISA KitCTLA4 适用: 小鼠 Colorimetric Sandwich ELISA 5-1200 pg/mL Cell Culture Supernatant, Plasma, Serum
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse CTLA4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each mouse CTLA4 standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: A37-F162
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))
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别名
- CTLA4
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背景
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Synonyms: Activation B7-1 antigen, B7, BB1, CD80, CD80_HUMAN, CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD80
Background: CTLA-4(Cytotoxic T-Lymphocyte Antigen 4), also known as CD152(Cluster of differentiation 152), is a protein receptor that down regulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Blockade of CTLA4 expression in vitro augmented HIV-specific CD4-positive T-cell function.
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基因ID
- 12477
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NCBI登录号
- NP_033973
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UniProt
- P09793
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途径
- Cancer Immune Checkpoints
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