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VEGFR2/CD309 ELISA 试剂盒

This 小鼠 VEGFR2/CD309 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 VEGFR2/CD309.
Rockland
产品编号 ABIN6719857
Supplier Product No.: koa0360
发货至: 中国

Quick Overview for VEGFR2/CD309 ELISA 试剂盒 (ABIN6719857)

抗原

See all VEGFR2/CD309 (VEGFR2) ELISA试剂盒
VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

62.5 pg/mL - 4000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum
  • 最低检测浓度

    62.5 pg/mL

    Supplier Product No.

    koa0360

    Supplier

    Rockland

    原理

    Mouse VEGFR2 - KDR Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant mouse VEGFR2. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 2 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse VEGFR2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. It is recommended that each mouse VEGFR2 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: A20-E762

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all VEGFR2/CD309 (VEGFR2) ELISA试剂盒

    VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

    别名

    VEGFR2/KDR

    背景

    Synonyms: CD309, Fetal liver kinase 1, FLK1, KDR, Kinase insert domain receptor, Kinase insert domain receptor (a type III receptor tyrosine kinase), Protein tyrosine kinase receptor Flk 1, Tyrosine kinase growth factor receptor, Vascular endothelial growth factor receptor 2, VEGFR, VEGFR 2, VEGFR2

    Background: Vascular endothelial growth factor receptor 2(VEGFR-2) is a VEGF receptor. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It is mapped to 4q12. This gene encodes one of the two receptors of the VEGF. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. VEGF receptor 2 and the adherens junction act as shear-stress cotransducers, mediating the transduction of shear-stress signals into vascular endothelial cells. In addition to those, VEGFR-2 is also a positive functional marker defining stem cells and distinguishing them from progenitors.

    基因ID

    16542

    NCBI登录号

    NP_034742

    UniProt

    P35918

    途径

    RTK signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling
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