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PLAT ELISA 试剂盒

This 人 PLAT ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 PLAT.
Rockland
产品编号 ABIN6719753
Supplier Product No.: koa0538
发货至: 中国

Quick Overview for PLAT ELISA 试剂盒 (ABIN6719753)

抗原

See all PLAT ELISA试剂盒
PLAT (Plasminogen Activator, Tissue (PLAT))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

31.2 pg/mL - 2000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
  • 最低检测浓度

    31.2 pg/mL

    Supplier Product No.

    koa0538

    Supplier

    Rockland

    原理

    Human PLAT - TPA Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant human PLAT. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human PLAT standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA, citrate) or urine to each empty well. It is recommended that each human PLAT standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: CHO, Immunogen sequence: M1-P562

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all PLAT ELISA试剂盒

    PLAT (Plasminogen Activator, Tissue (PLAT))

    别名

    PLAT/TPA

    背景

    Synonyms: Alteplase, Plasminogen activator tissue, Plasminogen activator tissue type, PLAT, Reteplase, t-PA, T-plasminogen activator, Tissue-type plasminogen activator chain B, tPA, TPA_HUMAN

    Background: Plasminogen activator, tissue, also called PLAT or TPA is a serine protease. This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This gene was mapped to 8p11.21. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding, decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism.

    基因ID

    5327

    NCBI登录号

    NP_000921

    UniProt

    P00750

    途径

    Autophagy, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, SARS-CoV-2 Protein Interactome
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