PLAT ELISA 试剂盒
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北京 101111
Quick Overview for PLAT ELISA 试剂盒 (ABIN1672825)
抗原
See all PLAT ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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原理
- Sandwich Human PLAT/TPA ELISA Kit to quantitate Human PLAT in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: CHO
Immunogen sequence: M1-P562Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Plasminogen Activator, Tissue (PLAT) ELISA Kit
PLAT 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Plasminogen Activator, Tissue (PLAT) ELISA KitPLAT 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Plasminogen Activator, Tissue (PLAT) ELISA KitPLAT 适用: 小鼠 Colorimetric Sandwich ELISA 39.063 pg/mL - 2500 pg/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator, Tissue (PLAT) ELISA KitPLAT 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator, Tissue (PLAT) ELISA KitPLAT 适用: Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Plasminogen Activator, Tissue (PLAT) ELISA KitPLAT 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma
Plasminogen Activator, Tissue (PLAT) ELISA KitPLAT 适用: 人 Colorimetric Sandwich ELISA 0.63 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum
Plasminogen Activator, Tissue (PLAT) ELISA KitPLAT 适用: 人 Colorimetric Sandwich ELISA 0.156-10 ng/mL Cell Culture Supernatant, Plasma, Serum
Plasminogen Activator, Tissue (PLAT) ELISA KitPLAT 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma
Plasminogen Activator, Tissue (PLAT) ELISA KitPLAT 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 6.4%, 6.5%, 4.5%
Inter-Assay Precision (CV%): 6.9%, 7.7%, 4.9% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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: "Mechanical Adaptability of the MMP-Responsive Film Improves the Functionality of Endothelial Cell Monolayer." in: Advanced healthcare materials, Vol. 6, Issue 14, (2018) (PubMed).
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- PLAT (Plasminogen Activator, Tissue (PLAT))
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别名
- PLAT
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背景
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Background: Plasminogen activator, tissue, also called PLAT or TPA is a serine protease. This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This gene was mapped to 8p11.21. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding, decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism.
Gene Full Name: plasminogen activator, tissue type
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基因ID
- 5327
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UniProt
- P00750
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途径
- Autophagy, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, SARS-CoV-2 Protein Interactome
抗原 See all PLAT ELISA试剂盒
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