PLAT ELISA 试剂盒
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- 抗原 See all PLAT ELISA试剂盒
- PLAT (Plasminogen Activator, Tissue (PLAT))
- 抗原表位
- AA 1-562
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human PLAT/TPA
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate), Urine
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: CHO
Immunogen sequence: M1-P562 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: CHO
Immunogen sequence: M1-P562 - Featured
- Discover our best selling PLAT ELISA Kit
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- Discover our top product PLAT ELISA Kit
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Plasminogen Activator, Tissue (PLAT) ELISA Kit
PLAT 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Plasminogen Activator, Tissue (PLAT) ELISA KitPLAT 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Plasminogen Activator, Tissue (PLAT) ELISA KitPLAT 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator, Tissue (PLAT) ELISA KitPLAT 适用: 小鼠 Colorimetric Sandwich ELISA 39.063 pg/mL - 2500 pg/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator, Tissue (PLAT) ELISA KitPLAT 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma
Plasminogen Activator, Tissue (PLAT) ELISA KitPLAT 适用: 小鼠 Colorimetric Sandwich ELISA 39.06 pg/mL - 2500 pg/mL Cell Culture Supernatant, Plasma, Serum
Plasminogen Activator, Tissue (PLAT) ELISA KitPLAT 适用: Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Plasminogen Activator, Tissue (PLAT) ELISA KitPLAT 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the peptidase S1 family.
Tissue Specificity: Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.
- 板类型
- Pre-coated
- 实验流程
- human PLAT ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PLAT has been precoated onto 96-well plates. Standards(CHO, M1-P562) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PLAT is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PLAT amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human PLAT standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA, citrate) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human PLAT standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 191, Standard deviation: 11.65, CV(%): 6.1
- Sample 2: n=16, Mean(pg/ml): 512, Standard deviation: 24.1, CV(%): 4.7
- Sample 3: n=16, Mean(pg/ml): 925, Standard deviation: 50, CV(%): 5.4,
- Sample 1: n=24, Mean(pg/ml): 244, Standard deviation: 17.57, CV(%): 7.2
- Sample 2: n=24, Mean(pg/ml): 732, Standard deviation: 42.5, CV(%): 5.8
- Sample 3: n=24, Mean(pg/ml): 1216, Standard deviation: 81.5, CV(%): 6.7
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "Mechanical Adaptability of the MMP-Responsive Film Improves the Functionality of Endothelial Cell Monolayer." in: Advanced healthcare materials, Vol. 6, Issue 14, (2018) (PubMed).
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: "Mechanical Adaptability of the MMP-Responsive Film Improves the Functionality of Endothelial Cell Monolayer." in: Advanced healthcare materials, Vol. 6, Issue 14, (2018) (PubMed).
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- 抗原 See all PLAT ELISA试剂盒
- PLAT (Plasminogen Activator, Tissue (PLAT))
- 别名
- PLAT (PLAT 产品)
- 别名
- T-PA ELISA Kit, TPA ELISA Kit, AU020998 ELISA Kit, AW212668 ELISA Kit, D8Ertd2e ELISA Kit, tPA ELISA Kit, PATISS ELISA Kit, tpa ELISA Kit, Plat ELISA Kit, plat ELISA Kit, t-pa ELISA Kit, plasminogen activator, tissue type ELISA Kit, plasminogen activator, tissue ELISA Kit, chromosome 20 open reading frame 181 ELISA Kit, plasminogen activator, tissue L homeolog ELISA Kit, PLAT ELISA Kit, Plat ELISA Kit, plat ELISA Kit, C20orf181 ELISA Kit, plat.L ELISA Kit
- 背景
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Protein Function: Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration.
Background: Plasminogen activator, tissue, also called PLAT or TPA is a serine protease. This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This gene was mapped to 8p11.21. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding, decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism.
Synonyms: Tissue-type plasminogen activator,t-PA,t-plasminogen activator,tPA,3.4.21.68,Alteplase,Reteplase,Tissue-type plasminogen activator chain A,Tissue-type plasminogen activator chain B,PLAT,
Full Gene Name: Tissue-type plasminogen activator
Cellular Localisation: Secreted, extracellular space. - 基因ID
- 5327
- UniProt
- P00750
- 途径
- Autophagy, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, SARS-CoV-2 Protein Interactome
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