PAI1 ELISA 试剂盒
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北京 101111
Quick Overview for PAI1 ELISA 试剂盒 (ABIN6719739)
抗原
See all PAI1 (SERPINE1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0520
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Supplier
- Rockland
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原理
- Human PAI-1 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: S22-P402 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA Kit
SERPINE1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Tissue Homogenate
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 兔 Colorimetric Competition ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 兔 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 马 Colorimetric Competition ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: Pig Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator Inhibitor 1 (SERPINE1) ELISA KitSERPINE1 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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说明
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Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human PAI-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, serum or plasma(heparin, EDTA, citrate ) to each empty well. It is recommended that each human PAI-1 standard solution and each sample be measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 6.1%, 7.7%, 7.7%
Inter-Assay Precision (CV%): 7.6%, 7.7%, 8.7% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))
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别名
- PAI-1
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背景
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Background: Plasminogen activator inhibitor-1 is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), the activators of plasminogen and hence fibrinolysis (the physiological breakdown of blood clots). It is a serine protease inhibitor (serpin) protein (SERPINE1). PAI-1 is mainly produced by the endothelium (cells lining blood vessels), but is also secreted by other tissue types, such as adipose tissue. The PAI-1 gene is located on the seventh chromosome (7q21.3-q22).1 Congenital deficiency of PAI-1 leads to a hemorrhagic diathesis (a tendency to hemorrhage). PAI-1 is present in increased levels in various disease states (such as a number of forms of cancer), as well as in obesity and the metabolic syndrome. It has been linked to the increased occurrence of thrombosis in patients with these conditions. Additionally, it has been shown to interact with ORM1.2 The standard product used in this kit is recombinant human PAI-1 with the molecular mass of 43KDa.
Gene Full Name: serpin family E member 1
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基因ID
- 5054
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NCBI登录号
- NP_000593
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UniProt
- P05121
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途径
- p53 Pathway, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration
抗原 See all PAI1 (SERPINE1) ELISA试剂盒
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