IL-10 ELISA 试剂盒
Quick Overview for IL-10 ELISA 试剂盒 (ABIN6719727)
抗原
See all IL-10 (IL10) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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Supplier Product No.
- koa0231
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Supplier
- Rockland
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原理
- Rat IL-10 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant rat IL-10. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 4 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Interleukin 10 (IL10) ELISA Kit
IL10 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 10 (IL10) ELISA KitIL10 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 10 (IL10) ELISA KitIL10 适用: 小鼠 AA 19-178 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Serum
Interleukin 10 (IL10) ELISA KitIL10 适用: 人 AA 19-178 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 10 (IL10) ELISA KitIL10 适用: 大鼠 AA 19-178 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum
Interleukin 10 (IL10) ELISA KitIL10 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 10 (IL10) ELISA KitIL10 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Ascitic Fluid, Cell Culture Supernatant, Cerebrospinal Fluid, Saliva, Serum, Urine
Interleukin 10 (IL10) ELISA KitIL10 适用: 人 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 10 (IL10) ELISA KitIL10 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 10 (IL10) ELISA KitIL10 适用: 兔 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat IL-10 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(EDTA) to each empty well. We recommend that each rat IL-10 standard solution and each sample is measured in duplicate.
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说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: S19-S178
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- IL-10 (IL10) (Interleukin 10 (IL10))
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别名
- IL-10
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背景
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Synonyms: CSIF, Cytokine synthesis inhibitory factor, GVHDS, IL 10, IL-10, IL10, IL10_HUMAN, IL10A, Interleukin 10, Interleukin-10, Interleukin10, MGC126450, MGC126451, T cell growth inhibitory factor, TGIF
Background: Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation. The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease. The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1.
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基因ID
- 25325
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NCBI登录号
- NP_036986
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UniProt
- P29456
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
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