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IL-10 ELISA 试剂盒

Name: IL-10 ELISA 试剂盒
SKU: ABIN411306
Availability: OutOfStock

The 人 IL-10 ELISA Kit (ABIN411306) is a Colorimetric ELISA Kit designed to quantify 人 IL-10. This ELISA Kit has been cited in 61 publications.

ELISA

1 image

(61 references)

产品编号 ABIN411306

发货至: 中国

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Quick Overview for IL-10 ELISA 试剂盒 (ABIN411306)

抗原

See all IL-10 (IL10) ELISA试剂盒

IL-10 (IL10) (Interleukin 10 (IL10))

抗原表位

AA 19-178

适用

人

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

7.8 pg/mL - 500 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Discover our top product IL10 ELISA Kit

最低检测浓度

7.8 pg/mL

原理

Sandwich Human IL-10/Interleukin-10 ELISA Kit to quantitate Human IL10 in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).

Analytical Method

Quantitative

特异性

Expression system for standard: E.coli
Immunogen sequence: S19-N178
Capture antibody and Detection antibody: monoclonal antibody from mouse, monoclonal antibody from mouse

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

< 0.5 pg/mL

组件
1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers
试剂未包括

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
Interleukin 10 (IL10) ELISA Kit
IL10 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 10 (IL10) ELISA Kit
IL10 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 10 (IL10) ELISA Kit
IL10 适用: 小鼠 AA 19-178 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum

Interleukin 10 (IL10) ELISA Kit
IL10 适用: 大鼠 AA 19-178 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum

Interleukin 10 (IL10) ELISA Kit
IL10 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 10 (IL10) ELISA Kit
IL10 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Ascitic Fluid, Cell Culture Supernatant, Cerebrospinal Fluid, Saliva, Serum, Urine

Interleukin 10 (IL10) ELISA Kit
IL10 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate

Interleukin 10 (IL10) ELISA Kit
IL10 适用: Cow Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 10 (IL10) ELISA Kit
IL10 适用: 小鸡 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 10 (IL10) ELISA Kit
IL10 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

样本量

100 μL

板类型

Pre-coated

实验流程

ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

实验精密度

Intra-Assay Precision (CV%): 6.4%, 4%, 6.5%
Inter-Assay Precision (CV%): 8.3%, 5.6%, 8.1%

限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

有效期

12 months
You, Tan, Dai, Chen, Xu, Yang, Zhang, Bai, Bie: "MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma." in: Oncotarget, Vol. 7, Issue 35, pp. 57099-57116, (2018) (PubMed).

He, Le, Frediani, Winterberg, Jin, Liverman, Hernandez, Cleeton, Vos: "Cardiometabolic risks vary by weight status in pediatric kidney and liver transplant recipients: A cross-sectional, single-center study in the USA." in: Pediatric transplantation, Vol. 21, Issue 6, (2018) (PubMed).

Badal, Kumar, Paul, Dayal, Bhansali, Bhadada, Kumar, Sachdeva et al.: "Peripheral blood mononuclear cells of patients with latent autoimmune diabetes secrete higher levels of pro- & anti-inflammatory cytokines compared to those with type-1 diabetes mellitus following in ..." in: The Indian journal of medical research, Vol. 145, Issue 6, pp. 767-776, (2018) (PubMed).

Dang, Xie, Zhou: "Expression of PYCARD gene transcript variant mRNA in peripheral blood mononuclear cells of primary gout patients with different Chinese medicine syndromes." in: Chinese journal of integrative medicine, Vol. 24, Issue 1, pp. 24-31, (2018) (PubMed).

Ma, Zhang, Zhao, Wang, Lin, Ma: "Expression of autophagy-related genes in cerebrospinal fluid of patients with tuberculous meningitis." in: Experimental and therapeutic medicine, Vol. 15, Issue 6, pp. 4671-4676, (2018) (PubMed).

Choghakhori, Abbasnezhad, Hasanvand, Amani: "Inflammatory cytokines and oxidative stress biomarkers in irritable bowel syndrome: Association with digestive symptoms and quality of life." in: Cytokine, Vol. 93, pp. 34-43, (2017) (PubMed).

Gheybi, Farrokhi, Ravanbod, Ostovar, Mehrzad, Nematollahi: "The correlation of CD19 + CD24 + CD38 + B cells and other clinicopathological variables with the proportion of circulating Tregs in breast cancer patients." in: Breast cancer (Tokyo, Japan), (2017) (PubMed).

Wang, Xie, Yang, Zhang, Wang, Wu, Shen, Xie: "Sulfated Cyclocarya paliurus polysaccharides markedly attenuates inflammation and oxidative damage in lipopolysaccharide-treated macrophage cells and mice." in: Scientific reports, Vol. 7, pp. 40402, (2017) (PubMed).

Li, Tang, Shuai, Jiang, Liu, Wang, Yao, Nie, Xie: "Mannose Receptor Mediates the Immune Response to Ganoderma atrum Polysaccharides in Macrophages." in: Journal of agricultural and food chemistry, Vol. 65, Issue 2, pp. 348-357, (2016) (PubMed).

Yao, Li, Wu, Zhang, Wang: "Inflammatory response of macrophages cultured with Helicobacter pylori strains was regulated by miR-155." in: International journal of clinical and experimental pathology, Vol. 8, Issue 5, pp. 4545-54, (2016) (PubMed).

Zhang, Li, Li, Shi, Zhou, Fu, Zhang, Yang, Fu, Lu: "Hypericin-photodynamic therapy induces human umbilical vein endothelial cell apoptosis." in: Scientific reports, Vol. 5, pp. 18398, (2016) (PubMed).

Zang, Jiang, Li, DU, Niu: "Characteristics of CD4+CD25+Foxp3+ regulatory T cells in patients with multiple organ dysfunction syndrome." in: Experimental and therapeutic medicine, Vol. 11, Issue 5, pp. 1908-1912, (2016) (PubMed).

Chen, Guo, Yang, Zheng, Zeng, Tong: "Protective effect of ginsenoside Rb1 on integrity of blood-brain barrier following cerebral ischemia." in: Experimental brain research, Vol. 233, Issue 10, pp. 2823-31, (2015) (PubMed).

Meng, Wang, Zhang, Qi, Liu, Wu: "Additive Suppression of LPS-Induced IL-10 and TNF-? by Pre-treatment of Dexamethasone and SB203580 in a Murine Alveolar Macrophage Cell Line (MH-S)." in: Inflammation, Vol. 38, Issue 3, pp. 1260-6, (2015) (PubMed).

Luo, Wang, Wang, Chen, Wang, Xu, Chen, Zeng: "Chronic NF-?B blockade improves renal angiotensin II type 1 receptor functions and reduces blood pressure in Zucker diabetic rats." in: Cardiovascular diabetology, Vol. 14, pp. 76, (2015) (PubMed).

Tang, Gao, Wang, Yuan, Ye, Chong, Xu, Jiang, Li, Yin, Miao, Wang, Jiang: "Apoptosis and anergy of T cell induced by pancreatic stellate cells-derived galectin-1 in pancreatic cancer." in: Tumour biology, Vol. 36, Issue 7, pp. 5617-26, (2015) (PubMed).

Berni Canani, Paparo, Nocerino, Cosenza, Pezzella, Di Costanzo, Capasso, Del Monaco, DArgenio, Greco, Salvatore: "Differences in DNA methylation profile of Th1 and Th2 cytokine genes are associated with tolerance acquisition in children with IgE-mediated cow's milk allergy." in: Clinical epigenetics, Vol. 7, Issue 1, pp. 38, (2015) (PubMed).

Dai, Wu, He, Zhang, Chen, Xu, Wang, Gruzdev, Zeldin, Wang: "Epoxyeicosatrienoic acids regulate macrophage polarization and prevent LPS-induced cardiac dysfunction." in: Journal of cellular physiology, Vol. 230, Issue 9, pp. 2108-19, (2015) (PubMed).

Zhang, Zhang, Li, Huang, Xu, Chu, Chen, Chen: "Ameliorative effects of Gualou Guizhi decoction on inflammation in focal cerebral ischemic-reperfusion injury." in: Molecular medicine reports, Vol. 12, Issue 1, pp. 988-94, (2015) (PubMed).

Liu, An, Liu, Li, Tang, Chen: "Mouse lung slices: An ex vivo model for the evaluation of antiviral and anti-inflammatory agents against influenza viruses." in: Antiviral research, Vol. 120, pp. 101-11, (2015) (PubMed).

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抗原 See all IL-10 (IL10) ELISA试剂盒

IL-10 (IL10) (Interleukin 10 (IL10))

别名

IL10

背景

Background: Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6KDa.
Gene Full Name: interleukin 10

基因ID

3586

UniProt

P22301

途径

Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints