Kallikrein 1 ELISA 试剂盒
Quick Overview for Kallikrein 1 ELISA 试剂盒 (ABIN6719700)
抗原
See all Kallikrein 1 (KLK1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0543
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Supplier
- Rockland
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原理
- Human KLK1 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human KLK1. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Kallikrein 1 (KLK1) ELISA Kit
KLK1 适用: 人 Colorimetric Competition ELISA 74.07 pg/mL - 6000 pg/mL Plasma, Serum
Kallikrein 1 (KLK1) ELISA KitKLK1 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Kallikrein 1 (KLK1) ELISA KitKLK1 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Kallikrein 1 (KLK1) ELISA KitKLK1 适用: 小鼠 Colorimetric Competition ELISA 49.4 pg/mL - 4000 pg/mL Plasma, Serum
Kallikrein 1 (KLK1) ELISA KitKLK1 适用: 大鼠 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Plasma, Serum, Tissue Homogenate
Kallikrein 1 (KLK1) ELISA KitKLK1 适用: 小鼠 AA 25-261 Colorimetric Sandwich ELISA
Kallikrein 1 (KLK1) ELISA KitKLK1 适用: 人 AA 25-262 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate
Kallikrein 1 (KLK1) ELISA KitKLK1 适用: 小鼠 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Kallikrein 1 (KLK1) ELISA KitKLK1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human KLK1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human KLK1 standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: I25-S262
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Kallikrein 1 (KLK1)
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别名
- KLK1
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背景
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Synonyms: Glandular kallikrein 1, hK1, Kallikrein 1 renal/pancreas/salivary, Kallikrein serine protease 1, Kallikrein-1, Kidney/pancreas/salivary gland kallikrein, KLK1, KLK1_HUMAN, Klk6, KLKR, Tissue kallikrein
Background: Kallikrein-1, also known as tissue kallikrein, is a protein that in humans is encoded by the KLK1 gene. This serine protease generates Lys-bradykinin by specific proteolysis of kininogen-1. KLK1 is a member of the peptidase S1 family. Its gene is mapped to 19q13.3. In all, it has got 262-amino acids which contain a putative signal peptide, followed by a short activating peptide and the protease domain. The protein is mainly found in kidney, pancreas, and salivary gland, showing a unique pattern of tissue-specific expression relative to other members of the family. KLK1 is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.
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基因ID
- 3816
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NCBI登录号
- NP_002248
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UniProt
- P06870
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途径
- Complement System
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