Kallikrein 1 ELISA 试剂盒
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- 抗原 See all Kallikrein 1 (KLK1) ELISA试剂盒
- Kallikrein 1 (KLK1)
- 抗原表位
- AA 25-262
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human KLK1
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Tissue Homogenate, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: I25-S262 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: I25-S262 - Featured
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Kallikrein 1 (KLK1) ELISA Kit
KLK1 适用: 人 Colorimetric Competition ELISA 74.07 pg/mL - 6000 pg/mL Plasma, Serum
Kallikrein 1 (KLK1) ELISA KitKLK1 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Kallikrein 1 (KLK1) ELISA KitKLK1 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Kallikrein 1 (KLK1) ELISA KitKLK1 适用: 小鼠 Colorimetric Competition ELISA 49.4 pg/mL - 4000 pg/mL Plasma, Serum
Kallikrein 1 (KLK1) ELISA KitKLK1 适用: 大鼠 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Plasma, Serum, Tissue Homogenate
Kallikrein 1 (KLK1) ELISA KitKLK1 适用: 小鼠 AA 25-261 Colorimetric Sandwich ELISA
Kallikrein 1 (KLK1) ELISA KitKLK1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Kallikrein 1 (KLK1) ELISA KitKLK1 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate
Kallikrein 1 (KLK1) ELISA KitKLK1 适用: 小鼠 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the peptidase S1 family. Kallikrein subfamily.
Tissue Specificity: Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84. .
- 板类型
- Pre-coated
- 实验流程
- human KLK1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK1 has been precoated onto 96-well plates. Standards(NSO, I25-S262) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human KLK1 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human KLK1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human KLK1 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 1468, Standard deviation: 67.53, CV(%): 4.6
- Sample 2: n=16, Mean(pg/ml): 3882, Standard deviation: 260.1, CV(%): 6.7
- Sample 3: n=16, Mean(pg/ml): 6352, Standard deviation: 349.4, CV(%): 5.5,
- Sample 1: n=24, Mean(pg/ml): 1625, Standard deviation: 95.9, CV(%): 5.9
- Sample 2: n=24, Mean(pg/ml): 4190, Standard deviation: 301.7, CV(%): 7.2
- Sample 3: n=24, Mean(pg/ml): 6526, Standard deviation: 417.7, CV(%): 6.4
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all Kallikrein 1 (KLK1) ELISA试剂盒
- Kallikrein 1 (KLK1)
- 别名
- KLK1 (KLK1 产品)
- 别名
- KLKR ELISA Kit, Klk6 ELISA Kit, hK1 ELISA Kit, klkr ELISA Kit, kallikrein ELISA Kit, 0610007D04Rik ELISA Kit, Kal ELISA Kit, Klk1b6 ELISA Kit, mGk-6 ELISA Kit, kallikrein 1 ELISA Kit, kallikrein 1 L homeolog ELISA Kit, KLK1 ELISA Kit, klk1.L ELISA Kit, LOC100101620 ELISA Kit, Klk1 ELISA Kit
- 背景
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Protein Function: Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Background: Kallikrein-1, also known as tissue kallikrein, is a protein that in humans is encoded by the KLK1 gene. This serine protease generates Lys-bradykinin by specific proteolysis of kininogen-1. KLK1 is a member of the peptidase S1 family. Its gene is mapped to 19q13.3. In all, it has got 262-amino acids which contain a putative signal peptide, followed by a short activating peptide and the protease domain. The protein is mainly found in kidney, pancreas, and salivary gland, showing a unique pattern of tissue-specific expression relative to other members of the family. KLK1 is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.
Synonyms: Kallikrein-1,3.4.21.35,Kidney/pancreas/salivary gland kallikrein,Tissue kallikrein,KLK1,
Full Gene Name: Kallikrein-1
- 基因ID
- 3816
- UniProt
- P06870
- 途径
- Complement System
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