IL1RN IQ-ELISA 试剂盒 (Interleukin 1 Receptor Antagonist)

Details for Product IL1RN IQ-ELISA Kit No. ABIN6384608
抗原
  • DIRA
  • ICIL-1RA
  • IL-1RN
  • IL-1ra
  • IL-1ra3
  • IL1F3
  • IL1RA
  • IRAP
  • MVCD4
  • IRAP1
  • IL1RN
  • IL-1RA
  • F630041P17Rik
  • interleukin 1 receptor antagonist
  • interleukin 1 receptor antagonist-like
  • IL1RN
  • Il1rn
  • IL1RNL
适用
小鼠
其他选择
Kits with alternative reactivity to:
6
6
6
6
5
5
5
4
3
2
2
2
2
1
1
检测方法
qPCR
实验类型
Sandwich ELISA
应用范围
ELISA
选项
原理 Mouse Immunoquantitative (PCR-Based) IL-1 Ra ELISA Kit for cell culture supernatants, plasma, and serum samples.
样品类型 Cell Culture Supernatant, Plasma, Serum
Analytical Method Semi-Quantitative
检测方法 qPCR
灵敏度 0.2 pg/mL
产品特性 IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.
组件
  • IL-1 Ra Microplate (Item A): 96 well PCR plate coated with anti-Mouse IL-1 Ra
  • Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
  • Standards (Item C): 2 vials of recombinant Mouse IL-1 Ra
  • Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
  • Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
  • Detection Affinity Reagent for IL-1 Ra (Item F): 2 vials of a 4x concentrated solution of anti-Mouse IL-1 Ra affinity reagent
  • IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
  • Primer Solution (Item I): 1.7 ml vial
  • PCR Master Mix (Item J): 1.2 ml vial
  • PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
  • Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer
试剂未包括
  • Real-time PCR instrument, Bio-Rad recommended
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • 100 mL and 1 liter graduated cylinders
  • Absorbent paper
  • Distilled or deionized water
  • Log-log graph paper or computer and software for data analysis
  • Tubes to prepare standard or sample dilutions
  • Heating block or water bath capable of 80°C
Plasmids, Primers & others Plasmids, Primers & others IL1RN products on genomics-online (e.g. as negative or positive controls)
抗原
别名 IL-1 Ra (IL1RN ELISA Kit 摘要)
基因ID 16181
UniProt P25085
途径 NF-kappaB Signaling, Hormone Transport, Cancer Immune Checkpoints
应用备注 The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.
说明

The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

样本量 25 µL
板类型 Pre-coated
实验流程
  1. Prepare all reagents, samples and standards as instructed
  2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C
  3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
  4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
  5. Add 15μL Primer solution 10μL of PCR master mix to each well
  6. Run real-time PCR
限制 仅限研究用
储存条件 4 °C,-20 °C,-80 °C
储存方法 May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
有效期 6 months
您还需要查找其他产品吗?