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NGFR ELISA 试剂盒

Name: NGFR ELISA 试剂盒
SKU: ABIN625340
Availability: OutOfStock

NGFR 适用: 人 Colorimetric Sandwich ELISA 80 pg/mL-20 ng/mL Cell Culture Supernatant, Plasma, Serum

ELISA

1 image

(1 reference)

产品编号 ABIN625340

发货至: 中国

Datasheet as PDF

抗原 See all NGFR ELISA试剂盒

NGFR (Nerve Growth Factor Receptor (NGFR))
适用
6

3

2

1
人

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

80 pg/mL-20 ng/mL

最低检测浓度

80 pg/mL

应用范围

ELISA

原理

Human NGFR (TNFRSF16) ELISA Kit for cell culture supernatants, plasma, and serum samples.

样品类型

Plasma, Cell Culture Supernatant, Serum

Analytical Method

Quantitative

特异性

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.

灵敏度

80 pg/mL

产品特性
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
组件
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
试剂未包括
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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应用备注

Recommended Dilution for serum and plasma samples2 fold

样本量

100 μL

板类型

Pre-coated

实验流程
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.
限制

仅限研究用
储存条件

-20 °C

储存方法

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

有效期

6 months
Chiang, Kan, Hsiao, Tang, Hsieh, Chiang, Tsai, Yeh, Hsieh, Liang, Chen, Yu: "Bone Marrow Stromal Antigen 2 Is a Novel Plasma Biomarker and Prognosticator for Colorectal Carcinoma: A Secretome-Based Verification Study." in: Disease markers, Vol. 2015, pp. 874054, (2015) (PubMed).
抗原 See all NGFR ELISA试剂盒

NGFR (Nerve Growth Factor Receptor (NGFR))

别名

NGF R (NGFR 产品)

别名

ngfr ELISA Kit, wu:fb38c02 ELISA Kit, wu:fc10h01 ELISA Kit, CD271 ELISA Kit, Gp80-LNGFR ELISA Kit, TNFRSF16 ELISA Kit, p75(NTR) ELISA Kit, p75NTR ELISA Kit, LNGFR ELISA Kit, RNNGFRR ELISA Kit, Tnfrsf16 ELISA Kit, p75 ELISA Kit, NRH1b ELISA Kit, fullback ELISA Kit, nrh1 ELISA Kit, p75ntr ELISA Kit, tnfrsf16 ELISA Kit, p75NGFR ELISA Kit, nerve growth factor receptor ELISA Kit, neurotrophin receptor associated death domain ELISA Kit, nerve growth factor receptor S homeolog ELISA Kit, nerve growth factor receptor (TNFR superfamily, member 16) ELISA Kit, NGFR ELISA Kit, nradd ELISA Kit, Ngfr ELISA Kit, ngfr.S ELISA Kit

背景

Gene Names: NGFR TNFRSF16
Protein names: Tumor necrosis factor receptor superfamily member 16 (Gp80-LNGFR) (Low affinity neurotrophin receptor p75NTR) (Low-affinity nerve growth factor receptor) (NGF receptor) (p75 ICD) (CD antigen CD271)

基因ID

4804

UniProt

P08138

途径

NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding