NGFR ELISA 试剂盒
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- 抗原 See all NGFR ELISA试剂盒
- NGFR (Nerve Growth Factor Receptor (NGFR))
- 抗原表位
- AA 29-250
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNFRSF16/NGFR
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
- Analytical Method
- Quantitative
- 特异性
- Sf21, K29-N250
- 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: sf21
Immunogen sequence: K29-N250 - Featured
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Nerve Growth Factor Receptor (NGFR) ELISA Kit
NGFR 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Nerve Growth Factor Receptor (NGFR) ELISA KitNGFR 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Nerve Growth Factor Receptor (NGFR) ELISA KitNGFR 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Nerve Growth Factor Receptor (NGFR) ELISA KitNGFR 适用: 人 Colorimetric Sandwich ELISA 80 pg/mL-20 ng/mL Cell Culture Supernatant, Plasma, Serum
Nerve Growth Factor Receptor (NGFR) ELISA KitNGFR 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- human TNFRSF16 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFRSF16 has been precoated onto 96-well plates. Standards (sf21, K29 - N250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF16 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFRSF16 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human TNFRSF16 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TNFRSF16 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 81, Standard deviation: 4.21, CV(%): 5.2
- Sample 2: n=16, Mean(pg/ml): 330, Standard deviation: 21.45, CV(%): 6.5
- Sample 3: n=16, Mean(pg/ml): 1560, Standard deviation: 90.48, CV(%): 5.8,
- Sample 1: n=24, Mean(pg/ml): 85, Standard deviation: 5.27, CV(%): 6.2
- Sample 2: n=24, Mean(pg/ml): 360, Standard deviation: 27, CV(%): 7.5
- Sample 3: n=24, Mean(pg/ml): 1350, Standard deviation: 90.45, CV(%): 6.7
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "Nerve growth factor and its receptor in schizophrenia." in: BBA clinical, Vol. 1, pp. 24-9, (2015) (PubMed).
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: "Nerve growth factor and its receptor in schizophrenia." in: BBA clinical, Vol. 1, pp. 24-9, (2015) (PubMed).
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- 抗原 See all NGFR ELISA试剂盒
- NGFR (Nerve Growth Factor Receptor (NGFR))
- 别名
- NGFR (NGFR 产品)
- 别名
- ngfr ELISA Kit, wu:fb38c02 ELISA Kit, wu:fc10h01 ELISA Kit, CD271 ELISA Kit, Gp80-LNGFR ELISA Kit, TNFRSF16 ELISA Kit, p75(NTR) ELISA Kit, p75NTR ELISA Kit, LNGFR ELISA Kit, RNNGFRR ELISA Kit, Tnfrsf16 ELISA Kit, p75 ELISA Kit, NRH1b ELISA Kit, fullback ELISA Kit, nrh1 ELISA Kit, p75ntr ELISA Kit, tnfrsf16 ELISA Kit, p75NGFR ELISA Kit, nerve growth factor receptor ELISA Kit, neurotrophin receptor associated death domain ELISA Kit, nerve growth factor receptor S homeolog ELISA Kit, nerve growth factor receptor (TNFR superfamily, member 16) ELISA Kit, NGFR ELISA Kit, nradd ELISA Kit, Ngfr ELISA Kit, ngfr.S ELISA Kit
- 背景
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Protein Function: Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver. .
Background: Nerve growth factor receptor (NGFR) is also referred to as p75(NTR) because of its ability to bind at low affinity not only to NGF, but also other neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5. The nerve growth factor receptor gene is at human chromosome region 17q12-17q22, distal to the chromosome 17 breakpoint in acute leukemias. The neurotrophin receptor p75(NTR), a tumor necrosis factor receptor superfamily member expressed in hepatic stellate cells after fibrotic and cirrhotic liver injury in humans, is a regulator of liver repair. In mice, depletion of p75(NTR) exacerbated liver pathology and inhibited hepatocyte proliferation in vivo. In addition, it is showed that neurotrophins activate p75(NTR) to induce apoptosis through the induction of the sphingomyelin (SM) cycle and increased production of ceramide. Overexpression of p75(NTR) is also found to activate the SM pathway.
Synonyms: Tumor necrosis factor receptor superfamily member 16,Gp80-LNGFR,Low affinity neurotrophin receptor p75NTR,Low-affinity nerve growth factor receptor,NGF receptor,p75 ICD,CD271,NGFR,TNFRSF16,
Full Gene Name: Tumor necrosis factor receptor superfamily member 16
Cellular Localisation: Membrane, Single-pass type I membrane protein. - 基因ID
- 4804
- UniProt
- P08138
- 途径
- NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding
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