电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

TYRO3 ELISA 试剂盒

This 人 TYRO3 ELISA Kit specifically detects TYRO3 in Cell Culture Supernatant 和 Serum.
产品编号 ABIN625289
发货至: 中国
Contact our Customer Service for availability and price in your country. Contact Info

Our Local Distributor

中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for TYRO3 ELISA 试剂盒 (ABIN625289)

抗原

See all TYRO3 ELISA试剂盒
TYRO3 (TYRO3 Protein Tyrosine Kinase (TYRO3))

适用

  • 4
  • 4
  • 1
  • 1
  • 1

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

40-10,000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum
  • 最低检测浓度

    40 pg/mL

    原理

    Human Dtk ELISA Kit for cell culture supernatants, plasma, and serum samples.

    Analytical Method

    Quantitative

    产品特性

    Custom Human Dtk ELISA Kit available on demand

    组件

    Pre-Coated 96-Well Strip Microplate
    Wash Buffer
    Stop Solution
    Assay Diluent(s)
    Lyophilized Standard
    Biotinylated Detection Antibody
    Streptavidin-Conjugated HRP
    TMB One-Step Substrate

    试剂未包括

    Distilled or deionized water
    100 mL and 1 liter graduated cylinders
    Tubes to prepare sample dilutions
    Protease and Phosphatase inhibitors
    Precision pipettes to deliver 2 μL to 1 mL volumes
    Adjustable 1-25 mL pipettes for reagent preparation
    Benchtop rocker or shaker
    Microplate reader capable of measuring absorbance at 450 nm
  • 样本量

    100 μL

    板类型

    Pre-coated

    实验流程

    Prepare all reagents, samples and standards as instructed in the manual.
    Add 100 µl of standard or sample to each well.
    Incubate 2.5 h at RT or O/N at 4°C.
    Add 100 µl of prepared biotin antibody to each well.
    Incubate 1 h at RT.
    Add 100 µl of prepared Streptavidin solution to each well.
    Incubate 45 min at RT.
    Add 100 µl of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 µl of Stop Solution to each well.
    Read at 450 nm immediately.

    限制

    仅限研究用
  • 储存条件

    4 °C

    有效期

    6 months
  • 抗原 See all TYRO3 ELISA试剂盒

    TYRO3 (TYRO3 Protein Tyrosine Kinase (TYRO3))

    别名

    Dtk

    途径

    RTK signaling
You are here: