TYRO3 ELISA 试剂盒
Quick Overview for TYRO3 ELISA 试剂盒 (ABIN625289)
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See all TYRO3 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 40 pg/mL
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原理
- Human Dtk ELISA Kit for cell culture supernatants, plasma, and serum samples.
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Analytical Method
- Quantitative
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产品特性
- Custom Human Dtk ELISA Kit available on demand
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组件
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Pre-Coated 96-Well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate -
试剂未包括
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Distilled or deionized water
100 mL and 1 liter graduated cylinders
Tubes to prepare sample dilutions
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 μL to 1 mL volumes
Adjustable 1-25 mL pipettes for reagent preparation
Benchtop rocker or shaker
Microplate reader capable of measuring absorbance at 450 nm
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TYRO3 Protein Tyrosine Kinase (TYRO3) ELISA Kit
TYRO3 适用: 人 Colorimetric Sandwich ELISA 0.45 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
TYRO3 Protein Tyrosine Kinase (TYRO3) ELISA KitTYRO3 适用: 小鼠 AA 31-418 Colorimetric Sandwich ELISA
TYRO3 Protein Tyrosine Kinase (TYRO3) ELISA KitTYRO3 适用: 人 AA 41-428 Colorimetric Sandwich ELISA
TYRO3 Protein Tyrosine Kinase (TYRO3) ELISA KitTYRO3 适用: 小鼠 Colorimetric Sandwich ELISA 0.4-100 ng/mL Cell Culture Supernatant, Plasma, Serum
TYRO3 Protein Tyrosine Kinase (TYRO3) ELISA KitTYRO3 适用: 小鼠 Colorimetric Sandwich ELISA 125-8000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately. -
限制
- 仅限研究用
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储存条件
- 4 °C
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有效期
- 6 months
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- TYRO3 (TYRO3 Protein Tyrosine Kinase (TYRO3))
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别名
- Dtk
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途径
- RTK signaling
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