TYRO3 ELISA 试剂盒
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- 抗原 See all TYRO3 ELISA试剂盒
- TYRO3 (TYRO3 Protein Tyrosine Kinase (TYRO3))
- 抗原表位
- pTyr
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human Phosphotyrosine DTK ELISA Kit. This assay semi-quantitatively measures phosphotyrosine DTK in lysate samples.
- 样品类型
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-DTK.
- 产品特性
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- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Biotinylated Anti-Phosphotyrosine Antibody
- Stop Solution
- Assay Diluent(s)
- Positive Control Sample
- Lysis Buffer
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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TYRO3 Protein Tyrosine Kinase (TYRO3) ELISA Kit
TYRO3 适用: 人 Colorimetric Sandwich ELISA 0.45 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
TYRO3 Protein Tyrosine Kinase (TYRO3) ELISA KitTYRO3 适用: 人 AA 41-428 Colorimetric Sandwich ELISA
TYRO3 Protein Tyrosine Kinase (TYRO3) ELISA KitTYRO3 适用: 小鼠 AA 31-418 Colorimetric Sandwich ELISA
TYRO3 Protein Tyrosine Kinase (TYRO3) ELISA KitTYRO3 适用: 人 Colorimetric Sandwich ELISA 40-10,000 pg/mL Cell Culture Supernatant, Serum
TYRO3 Protein Tyrosine Kinase (TYRO3) ELISA KitTYRO3 适用: 小鼠 Colorimetric Sandwich ELISA 0.4-100 ng/mL Cell Culture Supernatant, Plasma, Serum
TYRO3 Protein Tyrosine Kinase (TYRO3) ELISA KitTYRO3 适用: 小鼠 Colorimetric Sandwich ELISA 125-8000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- 有效期
- 6 months
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- 抗原 See all TYRO3 ELISA试剂盒
- TYRO3 (TYRO3 Protein Tyrosine Kinase (TYRO3))
- 别名
- DTK (TYRO3 产品)
- 别名
- TYRO3 ELISA Kit, brt ELISA Kit, byk ELISA Kit, dtk ELISA Kit, rse ELISA Kit, sky ELISA Kit, tif ELISA Kit, Xksy ELISA Kit, MGC145734 ELISA Kit, DKFZp469I0432 ELISA Kit, LOC100231598 ELISA Kit, BYK ELISA Kit, Dtk ELISA Kit, RSE ELISA Kit, Sky ELISA Kit, Tif ELISA Kit, Brt ELISA Kit, REK ELISA Kit, ksy ELISA Kit, xksy ELISA Kit, AI323366 ELISA Kit, Etk-2 ELISA Kit, Rse ELISA Kit, TYRO3 protein tyrosine kinase ELISA Kit, TYRO3 protein tyrosine kinase L homeolog ELISA Kit, TYRO3 protein tyrosine kinase 3 ELISA Kit, TYRO3 ELISA Kit, tyro3 ELISA Kit, Tyro3 ELISA Kit, tyro3.L ELISA Kit
- 基因ID
- 12446
- UniProt
- Q06418
- 途径
- RTK signaling
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