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TGFA ELISA 试剂盒

The 人 TGFA ELISA Kit (ABIN625093) is a Colorimetric ELISA Kit designed to quantify 人 TGFA. This ELISA Kit has been cited in 8 publications.

ELISA

1 image

(8 references)

产品编号 ABIN625093

发货至: 中国

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中国
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Quick Overview for TGFA ELISA 试剂盒 (ABIN625093)

抗原

See all TGFA ELISA试剂盒

TGFA (Transforming Growth Factor, alpha (TGFA))

适用

人

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

3 pg/mL - 1000 pg/mL

应用范围

ELISA

样品类型

Plasma, Cell Culture Supernatant, Serum

Discover our top product TGFA ELISA Kit

最低检测浓度

3 pg/mL

原理

Human TGF alpha ELISA Kit for cell culture supernatants, plasma, and serum samples.

Analytical Method

Quantitative

特异性

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ALCAM, Angiopoietin-1, Angiostatin, B7-1, BMP-7, CD30, CD40, CD40 Ligand, CTLA-4, CXCL16, Dkk-4 , DR6, Endostatin, E-Selectin, HB-EGF, ICAM-2, IGF-II, IL-10 Ra, IL-10 Rb, IL-13 Ra1, IL-18 BPa, IL-18 Rb, IL-18, IL-2 Ra, MMP-1, IL-2 Rb, IL-2 Rg, IL-21 R, MMP-2 , MMP-3, MMP-10, MMP-13).

产品特性
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
组件
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
,
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
试剂未包括
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Transforming Growth Factor, alpha (TGFA) ELISA Kit
TGFA 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Transforming Growth Factor, alpha (TGFA) ELISA Kit
TGFA 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Transforming Growth Factor, alpha (TGFA) ELISA Kit
TGFA 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Transforming Growth Factor, alpha (TGFA) ELISA Kit
TGFA 适用: 人 AA 40-89 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Serum

Transforming Growth Factor, alpha (TGFA) ELISA Kit
TGFA 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

Transforming Growth Factor, alpha (TGFA) ELISA Kit
TGFA 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

Transforming Growth Factor, alpha (TGFA) ELISA Kit
TGFA 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

Transforming Growth Factor, alpha (TGFA) ELISA Kit
TGFA 适用: Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

Transforming Growth Factor, alpha (TGFA) ELISA Kit
Small Sample TGFA 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Transforming Growth Factor, alpha (TGFA) ELISA Kit
TGFA 适用: Cow Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

应用备注

Suggested Plasma Dilution: 2 fold

板类型

Pre-coated

实验流程
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.
限制

仅限研究用
储存条件

-20 °C

储存方法

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

有效期

6 months
Lim, Yoo, Kim, Hur, Lee, Hur, Lee, Lee, Park, Lee, Chang, Kim, Kang, Hong, Kim, Kim, Yoon, Nam, Yang, Kim, Cho, Won: "GC1118, an Anti-EGFR Antibody with a Distinct Binding Epitope and Superior Inhibitory Activity against High-Affinity EGFR Ligands." in: Molecular cancer therapeutics, Vol. 15, Issue 2, pp. 251-63, (2016) (PubMed).

Koob, Lim, Zabek, Massee: "Cytokines in single layer amnion allografts compared to multilayer amnion/chorion allografts for wound healing." in: Journal of biomedical materials research. Part B, Applied biomaterials, Vol. 103, Issue 5, pp. 1133-40, (2015) (PubMed).

Briana, Liosi, Gourgiotis, Boutsikou, Marmarinos, Baka, Hassiakos, Malamitsi-Puchner: "Fetal concentrations of the growth factors TGF-α and TGF-β1 in relation to normal and restricted fetal growth at term." in: Cytokine, Vol. 60, Issue 1, pp. 157-61, (2013) (PubMed).

Miyabayashi, Ijichi, Mohri, Tada, Yamamoto, Asaoka, Ikenoue, Tateishi, Nakai, Isayama, Morishita, Omata, Moses, Koike: "Erlotinib prolongs survival in pancreatic cancer by blocking gemcitabine-induced MAPK signals." in: Cancer research, Vol. 73, Issue 7, pp. 2221-34, (2013) (PubMed).

Drzymała-Czyż, Banasiewicz, Tubacka, Majewski, Biczysko, Kościński, Drews, Walkowiak: "Inulin supplementation in rat model of pouchitis." in: Acta biochimica Polonica, Vol. 58, Issue 3, pp. 381-4, (2012) (PubMed).

Franzke, Cobzaru, Triantafyllopoulou, Löffek, Horiuchi, Threadgill, Kurz, van Rooijen, Bruckner-Tuderman, Blobel: "Epidermal ADAM17 maintains the skin barrier by regulating EGFR ligand-dependent terminal keratinocyte differentiation." in: The Journal of experimental medicine, Vol. 209, Issue 6, pp. 1105-19, (2012) (PubMed).

Pinto, Di Fabio, Maiello, Pini, Latiano, Aschele, Garufi, Bochicchio, Rosati, Aprile, Giaquinta, Torri, Bardelli, Gion, Martoni: "Phase II study of panitumumab, oxaliplatin, 5-fluorouracil, and concurrent radiotherapy as preoperative treatment in high-risk locally advanced rectal cancer patients (StarPan/STAR-02 Study)." in: Annals of oncology : official journal of the European Society for Medical Oncology, Vol. 22, Issue 11, pp. 2424-30, (2011) (PubMed).

Acocella, Bertrand, Beytout, Durrande, Garcia Rodriguez, Kosmidis, Micoud, Rey, Rodriguez Zapata, Roux: "Comparison of three different regimens in the treatment of acute brucellosis: a multicenter multinational study." in: The Journal of antimicrobial chemotherapy, Vol. 23, Issue 3, pp. 433-9, (1989) (PubMed).
抗原 See all TGFA ELISA试剂盒

TGFA (Transforming Growth Factor, alpha (TGFA))

别名

TGF-alpha

背景

Gene Symbols: TGFA
Protein Name (Synonyms): Protransforming growth factor alpha [Cleaved into: Transforming growth factor alpha (TGF-alpha) (EGF-like TGF) (ETGF) (TGF type 1)

基因ID

7039

UniProt

P01135

途径

NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway