IGFBP5 ELISA 试剂盒
(3 references)-
- 抗原 See all IGFBP5 ELISA试剂盒
- IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 2-800 ng/mL
- 最低检测浓度
- 2 ng/mL
- 应用范围
- ELISA
- 原理
- Human IGFBP-5 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, -2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
- 灵敏度
- 2 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our top product IGFBP5 ELISA Kit
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Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA Kit
IGFBP5 适用: Cow Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA KitIGFBP5 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA KitIGFBP5 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA KitIGFBP5 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA KitIGFBP5 适用: 大鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA KitIGFBP5 适用: 小鼠 Colorimetric Sandwich ELISA 5-3000 pg/mL Cell Culture Supernatant, Plasma, Serum
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA KitIGFBP5 适用: 小鼠 AA 20-271 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA KitIGFBP5 适用: 大鼠 AA 20-271 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA KitIGFBP5 适用: 犬 Colorimetric Competition ELISA 2.5-50 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA KitIGFBP5 适用: 大鼠, 小鼠 Colorimetric Sandwich ELISA 25-7.000 pg/mL Cell Culture Supernatant, Plasma, Serum
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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: "Serum concentrations of insulin-like growth factor-binding protein 5 in Crohn's disease." in: World journal of gastroenterology, Vol. 19, Issue 47, pp. 9049-56, (2013) (PubMed).
: "Serum concentrations of human insulin-like growth factor-1 and levels of insulin-like growth factor-binding protein-5 in patients with nonalcoholic fatty liver disease: association with liver ..." in: European journal of gastroenterology & hepatology, Vol. 24, Issue 3, pp. 255-61, (2012) (PubMed).
: "CDC's Model Performance Evaluation Program: assessment of the quality of laboratory performance for HIV-1 antibody testing." in: Public health reports (Washington, D.C. : 1974), Vol. 105, Issue 2, pp. 167-71, (1990) (PubMed).
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: "Serum concentrations of insulin-like growth factor-binding protein 5 in Crohn's disease." in: World journal of gastroenterology, Vol. 19, Issue 47, pp. 9049-56, (2013) (PubMed).
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- 抗原 See all IGFBP5 ELISA试剂盒
- IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))
- 别名
- IGFBP-5 (IGFBP5 产品)
- 别名
- IBP5 ELISA Kit, AI256729 ELISA Kit, AW208790 ELISA Kit, IGFBP-5 ELISA Kit, IGFBP-5P ELISA Kit, IGF-BP5 ELISA Kit, igfbp5 ELISA Kit, ibp5 ELISA Kit, IBP-5 ELISA Kit, xIGFBP-5 ELISA Kit, IGFBP5 ELISA Kit, igfbp5b ELISA Kit, zgc:165472 ELISA Kit, insulin like growth factor binding protein 5 ELISA Kit, insulin-like growth factor binding protein 5 ELISA Kit, insulin like growth factor binding protein 5 L homeolog ELISA Kit, insulin-like growth factor binding protein 5b ELISA Kit, insulin-like growth factor-binding protein 5 ELISA Kit, insulin-like growth factor binding protein 5a ELISA Kit, IGFBP5 ELISA Kit, Igfbp5 ELISA Kit, igfbp5.L ELISA Kit, igfbp5b ELISA Kit, igfbp5 ELISA Kit, LOC100194500 ELISA Kit, igfbp5a ELISA Kit
- 背景
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Gene Names: IGFBP5 IBP5
Protein names: Insulin-like growth factor-binding protein 5 (IBP-5) (IGF-binding protein 5) (IGFBP-5)
- 基因ID
- 3488
- UniProt
- P24593
- 途径
- WNT signaling, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding
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