ERK1 ELISA 试剂盒
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- 抗原 See all ERK1 (MAPK3) ELISA试剂盒
- ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.625 ng/mL - 40 ng/mL
- 最低检测浓度
- 0.625 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 0.249 ng/mL
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual - 样本量
- 100 µL
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12 months
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- 抗原 See all ERK1 (MAPK3) ELISA试剂盒
- ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))
- 别名
- Extracellular Signal Regulated Kinase 1 (ERK1) (MAPK3 产品)
- 别名
- ERK-1 ELISA Kit, ERK1 ELISA Kit, ERT2 ELISA Kit, HS44KDAP ELISA Kit, HUMKER1A ELISA Kit, P44ERK1 ELISA Kit, P44MAPK ELISA Kit, PRKM3 ELISA Kit, p44-ERK1 ELISA Kit, p44-MAPK ELISA Kit, Erk-1 ELISA Kit, Erk1 ELISA Kit, Ert2 ELISA Kit, Esrk1 ELISA Kit, Mnk1 ELISA Kit, Mtap2k ELISA Kit, Prkm3 ELISA Kit, p44 ELISA Kit, p44erk1 ELISA Kit, p44mapk ELISA Kit, ERK3 ELISA Kit, ERK6 ELISA Kit, P38GAMMA ELISA Kit, PRKM12 ELISA Kit, SAPK-3 ELISA Kit, SAPK3 ELISA Kit, fi06b09 ELISA Kit, wu:fi06b09 ELISA Kit, zERK1 ELISA Kit, Tb08.10J17.940 ELISA Kit, MAPK1 ELISA Kit, MNK1 ELISA Kit, AW123708 ELISA Kit, Erk6 ELISA Kit, P38gamma ELISA Kit, Prkm12 ELISA Kit, Sapk3 ELISA Kit, ATMAPK3 ELISA Kit, ATMPK3 ELISA Kit, T6D9.4 ELISA Kit, mitogen-activated protein kinase 3 ELISA Kit, mitogen-activated protein kinase 3 ELISA Kit, mitogen-activated protein kinase 12 ELISA Kit, mitogen activated protein kinase 3 ELISA Kit, mitogen-activated serine/threonine-protein kinase ELISA Kit, MAPK3 ELISA Kit, Mapk3 ELISA Kit, MAPK12 ELISA Kit, mapk3 ELISA Kit, Tc00.1047053509475.10 ELISA Kit, Tb927.8.3550 ELISA Kit, Mapk12 ELISA Kit, CEK1 ELISA Kit, MPK3 ELISA Kit
- 背景
- Alternative name: HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, MAPK3, ERT2, Mitogen-Activated Protein Kinase 3, Insulin-stimulated MAP2 kinase, Microtubule-associated protein 2 kinase
- 途径
- MAPK Pathway, RTK signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins
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