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MYD88 ELISA 试剂盒

MYD88 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN5690914
发货至: 中国
  • 抗原 See all MYD88 ELISA试剂盒
    MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))
    适用
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human MyD88 ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human MyD88.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    6 months
  • 抗原 See all MYD88 ELISA试剂盒
    MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))
    别名
    MyD88 (MYD88 产品)
    别名
    MYD88D ELISA Kit, XMyD88 ELISA Kit, myd88d ELISA Kit, MGC84928 ELISA Kit, CG2078 ELISA Kit, DMMYD88 ELISA Kit, DmMyD88 ELISA Kit, DmMyd88 ELISA Kit, Dmel\\CG2078 ELISA Kit, EP(2)2535 ELISA Kit, Kra ELISA Kit, LD20892 ELISA Kit, MYD88 ELISA Kit, MyD88 ELISA Kit, Myd88F ELISA Kit, dMyD88 ELISA Kit, dMyd88 ELISA Kit, kra ELISA Kit, myd88 ELISA Kit, zgc:103541 ELISA Kit, GB12344 ELISA Kit, NV10640 ELISA Kit, myd88-a ELISA Kit, myd88-b ELISA Kit, myeloid differentiation primary response 88 ELISA Kit, myeloid differentiation primary response 88 S homeolog ELISA Kit, myeloid differentiation primary response gene 88 ELISA Kit, CG2078 gene product from transcript CG2078-RB ELISA Kit, myeloid differentiation primary response protein MyD88-A ELISA Kit, myeloid differentiation primary response protein MyD88 ELISA Kit, myeloid differentiation factor 88 ELISA Kit, myeloid differentiation primary response 88 L homeolog ELISA Kit, MYD88 ELISA Kit, myd88.S ELISA Kit, Myd88 ELISA Kit, myd88 ELISA Kit, LOC413194 ELISA Kit, LOC100118553 ELISA Kit, LOC575153 ELISA Kit, myd88.L ELISA Kit
    基因ID
    4615
    UniProt
    Q99836
    途径
    NF-kappaB Signaling, TLR signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades
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